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Series GSE10627 Query DataSets for GSE10627
Status Public on Feb 25, 2008
Title Mll-AF9 induced changes in gene expression in various hematopoietic cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary The pathways by which oncogenes, such as MLL-AF9, initiate transformation and leukemia in humans and mice are incompletely defined. In a study of target cells and oncogene dosage, we found that Mll-AF9, when under endogenous regulatory control, efficiently transformed LSK (Lin- Sca1+ c-kit+) stem cells while committed granulocyte-monocyte progenitors (GMPs) were transformation-resistant and did not cause leukemia. Mll-AF9 was expressed at higher levels in hematopoietic stem (HSC) than GMP cells. Mll- AF9 gene dosage effects were directly shown in experiments where GMPs were efficiently transformed by the high dosage of Mll-AF9 resulting from retroviral transduction. Mll-AF9 up-regulated expression of 196 genes in both LSK and progenitor cells, but to higher levels in LSKs than in committed myeloid progenitors.
Keywords: mutant hematopoietic cells
 
Overall design Comparison of gene expression profiles among four types of hematopoietic cells (GMP, CMP, CLP and HSC), FACS sorted from wild type and Mll-AF9 knock-in mice. The goal was to identify genes differentially expressed in each Mll-AF9 cell type compared to the corresponding wild type cells.
 
Contributor(s) Kumar AR, Chen W, Sam TN, Wu B, Staggs RA, Hudson WA, Lund EA, Li Q, Kersey JH
Citation(s) 18455126
Submission date Feb 25, 2008
Last update date Feb 11, 2019
Contact name Ashish Kumar
E-mail kumar036@umn.edu
Organization name University of Minnesota
Street address 425 East River Road
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (51)
GSM267842 Wild type GMP-replicate 1
GSM267843 Wild type GMP-replicate 2
GSM267844 Wild type GMP-replicate 3
Relations
BioProject PRJNA107693

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10627_RAW.tar 286.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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