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Series GSE106229 Query DataSets for GSE106229
Status Public on Feb 03, 2018
Title Gene expression changes upon activation of RIG-I by a minimal potent RIG-I ligand
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have developed highly potent synthetic activators of the vertebrate immune system that specifically target the RIG-I receptor.  When introduced into mice, a family of short, triphosphorylated Stem Loop RNAs (SLRs) induces a potent interferon response and the activation of specific genes essential for antiviral defense. Using RNAseq, we provide the first in-vivo genome-wide view of the expression networks that are initiated upon RIG-I activation. We observe that SLRs specifically induce type I interferons, subsets of interferon-stimulated genes (ISGs), and cellular remodeling factors. By contrast, poly(I:C), which binds and activates multiple RNA sensors, induces type III interferons and several unique ISGs. The short length (10-14 base pairs) and robust function of SLRs in mice demonstrate that RIG-I forms active signaling complexes without oligomerizing on RNA. These findings demonstrate that SLRs are potent therapeutic and investigative tools for targeted modulation of the innate immune system.
Overall design We have triplicate samples of spleens from mice treated with SLR10, SLR14, poly(I:C) and pppNS, we have duplicate samples of mice treated with jetPEI vehicle control, and we have triplicate samples of untreated control mice
Contributor(s) Dickey TH, Molinari ES, Linehan MM, Fitzgerald ME, Potapova O, Iwasaki A, Pyle AM
Citation(s) 29492454
Submission date Oct 26, 2017
Last update date May 15, 2019
Contact name Anna Marie Pyle
Organization name Yale University
Street address 219 Prospect St.
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (17)
GSM2856224 SLR10-1
GSM2856225 SLR10-2
GSM2856226 SLR10-3
BioProject PRJNA418284
SRA SRP124941

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Supplementary file Size Download File type/resource
GSE106229_EdgeR_normalized_CPMs.csv.gz 1.7 Mb (ftp)(http) CSV
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