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| Status |
Public on Jun 04, 2020 |
| Title |
Transcriptional Analysis of Type II Alveolar Epithelial Cells in the Presence or Absence of Foxp3+ Regulatory T Cells during Experimental Acute Lung Injury Resolution |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
These studies profiled the expression of mRNA in lung type II alveolar epithelial cells during lipopolysacchride induced lung injury in the presence or absence of Foxp3+ Regulatory T Cells
Baseline, uninjured lung type II alveolar epithelial (AT2) cells or resolving AT2 cells, in the presence or absence of Foxp3+ regulatory T cells (Tregs), were sorted (7 days after intratracheal instillation of LPS for resolving conditions) and mRNAs differentially expressed (DE) between control AT2 cells, resolving AT2 cells with Tregs present, or resolving AT2 cells in mice depleted of Tregs were identified using microarrays.
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| Overall design |
Control lung type II alveolar epithelial (AT2) cells or two sets of AT2 cells obtained during a time point of lung injury resolution either in the presence or absence of regulatory T cells were sorted for mRNA analysis. mRNA expression was profiled using Mouse Gene 2.1 ST arrays (Affymetrix, Santa Clara, CA) which cover over 28,000 RefSeq transcripts
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| Contributor(s) |
Mock JR, Dang H, Doerschuk CM |
| Citation(s) |
32543909 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K12 HL119998 |
Application of Omics in Lung Disease |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Claire M Doerschuk |
| K08 HL129075 |
Regulatory T Cells Promote Alveolar Epithelial Repair |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Jason Robert Mock |
| R03 HL145255 |
Defining the Role of Regulatory T Cell-Derived MMP12 in Acute Lung Injury Resolution |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Jason Robert Mock |
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| Submission date |
Oct 24, 2017 |
| Last update date |
Sep 03, 2020 |
| Contact name |
Jason Robert Mock |
| E-mail(s) |
jason_mock@med.unc.edu
|
| Phone |
9199625347
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| Organization name |
University of North Carolina
|
| Department |
Internal Medicine
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| Street address |
125 Mason Farm Rd, Marsico Hall 7229G
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL22070 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript-level na35ens81] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA415567 |
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