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Series GSE1051 Query DataSets for GSE1051
Status Public on Jan 01, 2005
Title Seed development in LEAFY COTYLEDON1 mutants
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Analysis of gene expression in Ws-0 lec1 (LEAFY COTYLEDON1) mutant Arabidopsis thaliana. Developmental stages studied includes 24-Hr post-fertilization, globular stage, cotyledon stage, mature green stage, post-mature green stage, and seedlings.

Microarray Methods: Total RNA was extracted using the Hot Borate Method [Stones et. al. PNAS vol 98 no 20: 11806-11811 (2001)]. Biotinylated cRNA were prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit (Farmingdale, NY). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 4.0 (MAS 4.0) with a global scaling intensity set at 500.
Keywords: other
Contributor(s) Le BH, Bui AQ, Pelletier J, Kwong LW, Okamuro JK, Fischer RL, Drews GN, Harada JJ, Goldberg RB
Citation(s) 28739919
Submission date Feb 17, 2004
Last update date Jun 27, 2019
Contact name Bob Goldberg
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (2)
GPL71 [AG] Affymetrix Arabidopsis Genome Array
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (30)
GSM10445 WT 24-Hr Seed 1
GSM10446 WT 24-Hr Seed 2
GSM10448 WT Cotyledon Stage Seed 1
BioProject PRJNA87127

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Supplementary file Size Download File type/resource
GSE1051_RAW.tar 79.6 Mb (http)(custom) TAR (of CEL)

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