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| Status |
Public on Oct 18, 2017 |
| Title |
Genome wide mapping of chromatin binding sites of GATA2, GATA3, TFAP2A, TFAP2C transcription factors in trophoblast progenitors derived from human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Human embryonic stem cells (hESC) can be differentiated into progenitors resembling trophoblast upon exposure to BMP4. Among the earliest transcription factors that are activated after the BMP4 stimulation are GATA2, GATA3, TFAP2A and TFAP2C.
Using trophoblast progenitors at day 3 of BMP4-induced differentiation, here we profile the chromatin binding landscape of these 4 early transcription factors to analyse their putative targets and cross-connectivity in regualtion of trophoblast commitment.
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| Overall design |
hESC cell line H9 was differentiated towards trophoblast lineage using treatment with BMP4 for 3 days. The cells were used to perform ChIP using anti-GATA2, anti-GATA3, anti-TFAP2A and anti-TFAP2C antibodies followed by next generation sequencing.
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| Contributor(s) |
Shaposhnikov D, Straub T, Krendl C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 17, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Dmitry Shaposhnikov |
| E-mail(s) |
dmitry.shaposhnikov@helmholtz-muenchen.de
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| Organization name |
Helmholtz Center Munich
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| Street address |
Ingolstädter Landstraße 1
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| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (18)
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| GSM2817662 |
GATA2 ChIP, biological replicate 1 |
| GSM2817663 |
GATA2 ChIP, biological replicate 2 |
| GSM2817664 |
GATA2 ChIP, biological replicate 3 |
| GSM2817665 |
TFAP2A ChIP, biological replicate 1 |
| GSM2817666 |
TFAP2A ChIP, biological replicate 2 |
| GSM2817667 |
TFAP2A ChIP, biological replicate 3 |
| GSM2817668 |
TFAP2C ChIP, biological replicate 1 |
| GSM2817669 |
TFAP2C ChIP, biological replicate 2 |
| GSM2817670 |
TFAP2C ChIP, biological replicate 3 |
| GSM2817671 |
Input DNA for samples TFAP2A_1 and TFAP2C_2 |
| GSM2817672 |
Input DNA for sample TFAP2A_13 |
| GSM2817673 |
Input DNA for samples GATA3_8, GATA3_9, GATA2_5 |
| GSM2817674 |
Input DNA for samples TFAP2C_6 |
| GSM2817675 |
Input DNA for samples GATA3_9, GATA2_6 |
| GSM2817676 |
Input DNA for samples TFAP2A_10, GATA3_7, GATA2_4 |
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| Relations |
| BioProject |
PRJNA414606 |
| SRA |
SRP120153 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE105081_RAW.tar |
1.9 Gb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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