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Series GSE105081 Query DataSets for GSE105081
Status Public on Oct 18, 2017
Title Genome wide mapping of chromatin binding sites of GATA2, GATA3, TFAP2A, TFAP2C transcription factors in trophoblast progenitors derived from human embryonic stem cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Human embryonic stem cells (hESC) can be differentiated into progenitors resembling trophoblast upon exposure to BMP4. Among the earliest transcription factors that are activated after the BMP4 stimulation are GATA2, GATA3, TFAP2A and TFAP2C.
Using trophoblast progenitors at day 3 of BMP4-induced differentiation, here we profile the chromatin binding landscape of these 4 early transcription factors to analyse their putative targets and cross-connectivity in regualtion of trophoblast commitment.
Overall design hESC cell line H9 was differentiated towards trophoblast lineage using treatment with BMP4 for 3 days. The cells were used to perform ChIP using anti-GATA2, anti-GATA3, anti-TFAP2A and anti-TFAP2C antibodies followed by next generation sequencing.
Contributor(s) Shaposhnikov D, Straub T, Krendl C
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Submission date Oct 17, 2017
Last update date May 15, 2019
Contact name Dmitry Shaposhnikov
Organization name Helmholtz Center Munich
Street address Ingolstädter Landstraße 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM2817659 GATA3 ChIP, biological replicate 1
GSM2817660 GATA3 ChIP, biological replicate 2
GSM2817661 GATA3 ChIP, biological replicate 3
BioProject PRJNA414606
SRA SRP120153

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE105081_RAW.tar 1.9 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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