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Series GSE10502 Query DataSets for GSE10502
Status Public on Apr 24, 2008
Title Time course expression analysis of the iron deficiency (-Fe) response in Arabidopsis roots
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We performed a time course analysis (TC data set) of the response of whole seedling roots to -Fe at 6 time points after transfer (3, 6, 12, 24, 48, and 72 hours).
Little is known about how developmental cues affect the way cells interpret their environment. Here we characterize the transcriptional response of different cell layers and developmental stages of the Arabidopsis root to high salinity and find that transcriptional responses are highly constrained by developmental parameters. These transcriptional changes lead to the differential regulation of specific biological functions in subsets of cell-layers, several of which correspond to observable physiological changes. We show that known stress pathways primarily control semi-ubiquitous responses and use mutants that disrupt epidermal patterning to reveal cell-layer specific and inter-cell-layer effects. By performing a similar analysis using iron-deprivation we identify common cell-type specific stress responses and environment-independent biological functions that define each cell type.
Keywords: time course analysis
 
Overall design Seedlings were grown for 5 days before transfer to iron deficient (-Fe) conditions (0.3mM Ferrozine in MS media containing no iron sulfate). Whole roots were harvested at 6 time points after the transfer.
 
Contributor(s) Long TA, Wang JY, Benfey PN
Citation(s) 18436742
Submission date Feb 12, 2008
Last update date Aug 28, 2018
Contact name Terri Anita Long
E-mail(s) tlong@duke.edu
Phone 919-613-8202
Fax 919-613-8177
Organization name Duke University
Department Biology
Lab Philip Benfey
Street address Box 90338
City Durham
State/province NC
ZIP/Postal code 27707
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (14)
GSM265461 Arabidopsis, whole roots, standard conditions, rep 1
GSM265462 Arabidopsis, whole roots, standard conditions, rep 2
GSM265463 Arabidopsis, whole roots, -Fe, 3 hour, rep 1
This SubSeries is part of SuperSeries:
GSE10576 Iron deficiency (-Fe) effect: Arabidopsis roots
Relations
Affiliated with GSE69995
BioProject PRJNA108935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10502_RAW.tar 45.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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