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| Status |
Public on Mar 12, 2019 |
| Title |
Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor [Ring1b ChIP] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Regulation of transit amplifying cell formation from self-renewing stem cell is fundamental process for cell replacement in a controlled way. Here we analyse the properties of a population of mesenchymal TACs in the continuously growing mouse incisor to identify key components of the molecular regulation that drives proliferation. We show that the polycomb repressive complex 1 acts as a global regulator of the TAC phenotype by its direct action on the expression of key cell cycle regulatory genes and also by regulating Wnt/b-catenin signalling activity. We also identify an essential requirement for TACs in maintaining the mesenchymal stem cells, indicative of a positive feedback mechanism.
Analysing the properties of mesenchymal transit amplifying cells population and identifing key components of the molecular regulation that drives proliferation.
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| Overall design |
Ring1b ChIP-seq and input sample
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| Contributor(s) |
An Z, Akily B, Sabalic M, Zong G, Sharpe PT |
| Citation(s) |
29874594 |
| |
| Submission date |
Oct 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Paul T. Sharpe |
| Organization name |
King's College London
|
| Department |
Centre for Craniofacial and Regenerative Biology
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| Lab |
Sharpe Lab
|
| Street address |
Guy's hospitial
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| City |
London |
| State/province |
Please select one below: |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE104893 |
Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor |
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| Relations |
| BioProject |
PRJNA414105 |
| SRA |
SRP119852 |
