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Series GSE104765 Query DataSets for GSE104765
Status Public on Jun 30, 2019
Title Immunosubtyping of breast cancer reveals distinct myeloid cell biology and immunotherapy resistance mechanisms
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cancer-induced immune responses affect tumor progression and therapeutic response. In multiple murine models and clinical datasets, we identified large variations of neutrophils and macrophages, which define “immune subtypes” of triple negative breast cancer (TNBC) including neutrophil-enriched (NES) and macrophage-enriched subtypes (MES). Different tumor-intrinsic pathways and mutual regulation between macrophages/monocytes and neutrophils contribute to the development of dichotomous myeloid compartment. MES contains predominantly macrophages that are CCR2-dependent and exhibit variable responses to immune checkpoint blockade (ICB). NES exhibits systemic and local accumulation of immunosuppressive neutrophils (or granulocytic myeloid-derived suppressor cells (gMDSCs), is resistant to ICB, and contains a minority of macrophages that appear to be unaffected by CCR2 knockout. A MES-to-NES conversion mediated acquired ICB resistance of initially sensitive MES models. Our results demonstrate diverse myeloid cell frequencies, functionality, and potential roles in immunotherapies, and highlight the need to better understand the inter-patient heterogeneity of the myeloid compartment.
 
Overall design Transcritomic profiling of 1) eight different breast tumor cell lines cultured in vitro and 2) FACS-purified tumor-infiltrating neutrophils and macrophages from four breast tumors in vivo. Each sample was prepared in triplicate.
 
Contributor(s) Kim I, Zhang X, Mo Q, Tan Y
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Submission date Oct 10, 2017
Last update date Jun 30, 2019
Contact name Ik Sun Kim
E-mail(s) iskim@bcm.edu
Organization name Baylor College of Medicine
Department Lester and Sue Smith Breast Center
Lab Dr. Shawn Zhang's lab
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (48)
GSM2807334 T11_cancer_1
GSM2807335 T11_cancer_2
GSM2807336 T11_cancer_3
Relations
BioProject PRJNA413800
SRA SRP119677

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104765_RawCountTable.txt.gz 1.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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