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| Status |
Public on Oct 09, 2017 |
| Title |
miRNA expression microarrays in GM-CSF or GM-CSF+IL6-induced mouse MDSC |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Myeloid-derived suppressor cells (MDSCs) have emerged as major regulators of immune responses in cancer and other pathological conditions. Multiple factors including cytokines, transcription factors and multiple signaling pathways are involved in MDSC differentiation. Cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin(IL-6) etc could in vitro mediate development of MDSCs.IL-6 with GM-CSF mediated MDSC not only had stronger suppressive function but also the dynamics of their suppressive function was different from GM-CSF alone mediated MDSCs.To found a new regulatory factor (s) in tumor and inflammatory environments, we compared GM-CSF and IL-6 mediated MDSCs with GM-CSF alone mediated MDSCs using lncRNA microarray, miRNA microarrays and protein-coding mRNA microarrays.
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| Overall design |
Inflammatory factors associated MDSCs were generated by cultivating bone marrow cells of C57BL/6 mice in petri dishes for 4 days in the presence of 5% FBS medium containing GM-CSF (40 ng/ml) only, GM-CSF (40 ng/ml) plus IL-6 (40 ng/ml) using different processing group for each experiment.
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| Contributor(s) |
Mei S, Yang R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 08, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yunhuan Gao |
| E-mail(s) |
gaoyh_fei@163.com
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| Organization name |
Nankai University
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| Department |
School of Medicine
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| Lab |
317
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| Street address |
Wei Jin Road No. 94
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| City |
tianjin |
| ZIP/Postal code |
300071 |
| Country |
China |
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| Platforms (1) |
| GPL24099 |
Agilent-031184 Unrestricted Mouse miRNA V21 Microarray (miRNA version Grid_Date 16-Jan-2013) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA413631 |
