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Series GSE104690 Query DataSets for GSE104690
Status Public on Jan 11, 2018
Title A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb group proteins regulate self-renewal and
differentiation in many stem cell systems. When
assembled into two canonical complexes, PRC1
and PRC2, they sequentially deposit H3K27me3
and H2AK119ub histone marks and establish repressive
chromatin, referred to as Polycomb domains.
Non-canonical PRC1 complexes retain RING1/
RNF2 E3-ubiquitin ligases but have unique sets of
accessory subunits. How these non-canonical complexes
recognize and regulate their gene targets
remains poorly understood. Here, we show that
the BCL6 co-repressor (BCOR), a member of the
PRC1.1 complex, is critical for maintaining primed
pluripotency in human embryonic stem cells
(ESCs). BCOR depletion leads to the erosion of
Polycomb domains at key developmental loci and
the initiation of differentiation along endoderm
and mesoderm lineages. The C terminus of BCOR
regulates the assembly and targeting of the
PRC1.1 complex, while the N terminus contributes
to BCOR-PRC1.1 repressor function. Our findings
advance understanding of Polycomb targeting and
repression in ESCs and could apply broadly across
developmental systems.
 
Overall design Transcriptional Repression by the non-canonical PRC1.1 complex investigated by ChIP-Seq and RNA-Seq
 
Contributor(s) Wang Z, Gearhart MD, Bardwell VJ, Ivanova N
Citation(s) 29337181
Submission date Oct 06, 2017
Last update date May 15, 2019
Contact name Micah Gearhart
E-mail(s) gearh006@umn.edu
Organization name University of Minnesota
Department Genetics, Cell Biology and Development
Street address 6-160 Jackson Hall, 321 Church St.
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (42)
GSM2805865 Input DNA from Wild Type cells
GSM2805866 BCOR ChIP Sample from Wild Type cells
GSM2805867 KDM2B ChIP Sample from Wild Type cells
Relations
BioProject PRJNA413520
SRA SRP119523

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104690_BCOR_H1_k2bko_081817_broad_peaks.broadPeak.gz 379.1 Kb (ftp)(http) BROADPEAK
GSE104690_BCOR_H1_rnf2rminus_081817_broad_peaks.broadPeak.gz 922.9 Kb (ftp)(http) BROADPEAK
GSE104690_BCOR_H1_rnf2rplus_081817_broad_peaks.broadPeak.gz 968.4 Kb (ftp)(http) BROADPEAK
GSE104690_BCOR_H1_wt_081817_broad_peaks.broadPeak.gz 588.8 Kb (ftp)(http) BROADPEAK
GSE104690_BCOR_hESC_normalized_FPKM.csv.gz 7.3 Mb (ftp)(http) CSV
GSE104690_RAW.tar 6.5 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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