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Series GSE10469 Query DataSets for GSE10469
Status Public on May 10, 2009
Title Time course progression of BMP4-treated hESC gene expressions under 4 % and 20 % oxygen conditions.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary H1 hESC were exposed to BMP4 (10 ng/ml) in absence of FGF2 for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) conditions to identify candidate genes and gene networks associated with specification from totipotent precursor cells to trophoblast. RNA from cells under both conditions was isolated at each time point.
Approximately 32,000 probe sets (out of 40,391) provided a signal above background at one of the time points. Of these, 14,478 showed a ≥2-fold (plus or minus) change (p<0.05) under 4 % O2, and 18,580 under 20 % O2. The data indicate the potential usefulness of hESC grown in presence of BMP4 and absence of FGF2 for studying both the emergence of trophoblast from hESC precursors and for its subsequent differentiation over time, space and varied O2 concentrations.
Keywords: Genes and gene networks associated with specification from totipotent precursor cells to trophoblast
 
Overall design Twelve sets of H1 hESC RNA were prepared from BMP4 treated cells for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) and cultured on Matrigel coated six-well plate.
 
Contributor(s) Ezashi T, Schulz LC, Roberts M
Citation(s) 19541600, 23493551
Submission date Feb 11, 2008
Last update date Jan 23, 2019
Contact name Toshihiko Ezashi
E-mail(s) ezashit@missouri.edu
Phone 573 884-9601
Organization name University of Missouri-Columbia
Department Animal Sciences
Lab R.M. Roberts
Street address 240a CS Bond Life Sciences Center
City Columbia
State/province MO
ZIP/Postal code 65211
Country USA
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (12)
GSM264878 H1hESC 0h 4%O2
GSM264879 H1hESC 3h 4%O2
GSM264880 H1hESC 12h 4%O2
Relations
BioProject PRJNA108029

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10469_RAW.tar 77.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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