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| Status |
Public on Nov 16, 2017 |
| Title |
Non-monotonic changes in Progenitor Cell Behavior and Gene expression during aging of the Adult Neural Stem Cell Niche |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Neural stem cell activity in the ventricular-subventricular zone (V-SVZ) decreases with aging, thought to occur by a unidirectional decline. However, by analyzing the V-SVZ transcriptome of male mice at 2, 6, 18 and 22 months, we found that most of the genes that change significantly over time show a reversal of trend, with a maximum or minimum expression at 18 months. In vivo, MASH1+ progenitor cells decrease in number and proliferation between 2-18 months, but these increased between 18-22 months. Time-lapse lineage analysis of 944 V-SVZ cells showed that age-related declines in neurogenesis were recapitulated in vitro in clones. However, activated type B/type C cell clones divide slower from 2-18 months, then unexpectedly faster at 22 months, with impaired transition to type A neuroblasts. Our findings indicate that aging of the V-SVZ involves significant non-monotonic changes that are programed within progenitor cells, and observable independent of the aging niche.
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| Overall design |
RNA extracted from Mouse SVZ, in triplicate, from ages 2, 6, 18 and 22 months.
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| Contributor(s) |
Apostolopoulou M, Kiehl TR, Temple S |
| Citation(s) |
29129683 |
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| Submission date |
Oct 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Thomas R Kiehl |
| E-mail(s) |
tomkiehl@neuralsci.org
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| Phone |
518-694-8188
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| Organization name |
Neural Stem Cell Institute
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| Street address |
One Discovery Drive
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| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA413400 |
| SRA |
SRP119471 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104651_apostolopoulou-svz-rna-seq.xlsx |
4.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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