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Series GSE104651 Query DataSets for GSE104651
Status Public on Nov 16, 2017
Title Non-monotonic changes in Progenitor Cell Behavior and Gene expression during aging of the Adult Neural Stem Cell Niche
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Neural stem cell activity in the ventricular-subventricular zone (V-SVZ) decreases with aging, thought to occur by a unidirectional decline. However, by analyzing the V-SVZ transcriptome of male mice at 2, 6, 18 and 22 months, we found that most of the genes that change significantly over time show a reversal of trend, with a maximum or minimum expression at 18 months. In vivo, MASH1+ progenitor cells decrease in number and proliferation between 2-18 months, but these increased between 18-22 months. Time-lapse lineage analysis of 944 V-SVZ cells showed that age-related declines in neurogenesis were recapitulated in vitro in clones. However, activated type B/type C cell clones divide slower from 2-18 months, then unexpectedly faster at 22 months, with impaired transition to type A neuroblasts. Our findings indicate that aging of the V-SVZ involves significant non-monotonic changes that are programed within progenitor cells, and observable independent of the aging niche.
 
Overall design RNA extracted from Mouse SVZ, in triplicate, from ages 2, 6, 18 and 22 months.
 
Contributor(s) Apostolopoulou M, Kiehl TR, Temple S
Citation(s) 29129683
Submission date Oct 05, 2017
Last update date May 15, 2019
Contact name Thomas R Kiehl
E-mail tomkiehl@neuralsci.org
Phone 518-694-8188
Organization name Neural Stem Cell Institute
Street address One Discovery Drive
City Rensselaer
State/province NY
ZIP/Postal code 12144
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM2805059 2 Month SVZ 1
GSM2805060 2 Month SVZ 2
GSM2805061 2 Month SVZ 3
Relations
BioProject PRJNA413400
SRA SRP119471

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Supplementary file Size Download File type/resource
GSE104651_apostolopoulou-svz-rna-seq.xlsx 4.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data is available on Series record

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