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Series GSE104511 Query DataSets for GSE104511
Status Public on Feb 08, 2018
Title Circuit design features of a stable two-cell system
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cell communication within tissues is mediated by multiple paracrine signals including growth factors, which control cell survival and proliferation. Cells and the growth factors they produce and receive constitute a circuit, yet the design features of cell circuits involved in tissue homeostasis are unknown. Here we used computational and experimental approaches to characterize the features of cell circuits based on growth factor exchange between macrophages and fibroblasts, two cell types found in most mammalian tissues. We found that the macrophage-fibroblast cell circuit is stable and robust to perturbations. We employed analytical screening of all possible two-cell circuit topologies and defined the circuit features sufficient for stability, including environmental constraint and negative feedback regulation. Moreover, we discovered that cell-cell contact was essential for the stability of the macrophage-fibroblast circuit. These findings highlight general principles of cell circuit design, and provide a new perspective on quantitative understanding of tissue homeostasis.
Overall design 1 sample of murine embryonic fibroblast and 1 sample murine bone marrow derived macrophages are analyzed for their expression of growth factors and growth factor receptors
Contributor(s) Zhou X, Franklin RA, Medzhitov R
Citation(s) 29398113
Submission date Oct 02, 2017
Last update date May 15, 2019
Contact name Xu Zhou
Organization name Boston Children's Hospital
Department Pediatrics
Lab Xu Zhou
Street address 300 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM2802676 WT_MEF
GSM2802677 WT_BMDM
BioProject PRJNA412883
SRA SRP119265

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104511_MEF_BMDM_expression.csv.gz 809.8 Kb (ftp)(http) CSV
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