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| Status |
Public on Sep 01, 2018 |
| Title |
Metformin induces chromosome reorganization and changes in gene expression in normal human fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the effects of metformin treatment on the transcriptome of normal human dermal fibroblasts isolated from foreskin (designated 2DD). We sequenced mRNA from 2 replicates of proliferative (PRO) and 2DD cells treated with two concentrations of metformin (0.5mM and 1.0 mM;5 days exposure). Comparative analyses with PRO transcripts a baseline indicate that fibroblsts exposed to different concentration of metformin result in differnetial transcript profiles.
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| |
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| Overall design |
Examination of mRNAs from proliferative and metformin treated (0.5mM and 1.0 mM for 5 days) 2DD normal human dermal/foreskin fibroblasts.
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| |
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| Contributor(s) |
Gillespie ZE, MacKay KA, Kusalik A, Eskiw CH |
| Citation missing |
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| |
| Submission date |
Sep 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Christopher H. Eskiw |
| E-mail(s) |
c.eskiw@usask.ca
|
| Phone |
306-966-2454
|
| Organization name |
University of Saskatchewan
|
| Department |
Food and Bioproduct Science
|
| Lab |
Eskiw Lab
|
| Street address |
51 Campus Drive
|
| City |
Saskatoon |
| State/province |
SK |
| ZIP/Postal code |
S7N 5A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA412662 |
| SRA |
SRP119165 |
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