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Series GSE104276 Query DataSets for GSE104276
Status Public on Feb 10, 2018
Title Single-cell RNA-Seq reveals a developmental atlas of human prefrontal cortex
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian prefrontal cortex contains billions of cells, some of which are known as neurons to play critical roles in memory, cognitive ability, decision making, social behavior etc. through participating into complex neural circuits. Although neural circuits build up in the late stage of human embryo development and even after birth, the diverse and functional cells start to generate and migrate to the appropriate location play essential roles as basis for developing future circuits. However, it remains challenging to identify cell types of developing human PFC and distinguish their developmental features. Here, to address these challenges, single cells from human embryos PFC were carried out for RNA-seq. Detailed analysis of neural progenitor cells (NPCs) illustrated a developmental feature of intermediate progenitor (IP) cells and revealed new marker genes of IP cells. We also mapped the neurogenesis timeline of PFC excitatory neurons and intrinsic singles regulating neuron maturation and circuit formation. Our screening and characterization approach provides a blueprint of human PFC development in early and mid embryonic stages, with which to systematically discover the cellular basis and molecular regulation of PFC function in humans.
 
Overall design Here we performed single cell RNA-seq analysis for prefrontal cortex (PFC), covering the developmental stages of gestational weeks 8-26.
 
Contributor(s) Zhong S, Zhang S, Fan X, Wu Q, Wang X, Tang F
Citation(s) 29539641
Submission date Sep 26, 2017
Last update date Feb 27, 2019
Contact name Shu Zhang
E-mail zhshu0917@163.com
Phone +86-13031133622
Organization name BIOPIC
Lab Fuchou Tang
Street address Peking University, No.5 Yiheyuan Road Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100871
Country China
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (39)
GSM2884059 GW08_PFC1
GSM2884060 GW09_PFC1
GSM2884061 GW10_PFC1_1
Relations
BioProject PRJNA412220
SRA SRP118904

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104276_RAW.tar 36.9 Mb (http)(custom) TAR (of TXT)
GSE104276_all_pfc_2394_UMI_TPM_NOERCC.xls.gz 31.6 Mb (ftp)(http) XLS
GSE104276_all_pfc_2394_UMI_count_NOERCC.xls.gz 15.3 Mb (ftp)(http) XLS
GSE104276_readme_sample_barcode.xlsx 286.7 Kb (ftp)(http) XLSX
Raw data are available in SRA
Processed data provided as supplementary file

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