NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE104059 Query DataSets for GSE104059
Status Public on Feb 22, 2018
Title Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila
Organisms Drosophila melanogaster; Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Regulatory decisions in Drosophila require Polycomb group (PcG) proteins to maintain the silent state and Trithorax group (TrxG) proteins to oppose silencing. Since PcG and TrxG are ubiquitous and lack apparent sequence specificity, a long-standing model is that targeting occurs via protein interactions; for instance, between repressors and PcG proteins. Instead, we found that Pc-repressive complex 1 (PRC1) purifies with coactivators Fs(1)h [female sterile (1) homeotic] and Enok/Br140 during embryogenesis. Fs(1)h is a TrxG member and the ortholog of BRD4, a bromodomain protein that binds to acetylated histones and is a key transcriptional coactivator in mammals. Enok and Br140, another bromodomain protein, are orthologous to subunits of a mammalian MOZ/MORF acetyltransferase complex. Here we confirm PRC1-Br140 and PRC1-Fs(1)h interactions and identify their genomic binding sites. PRC1-Br140 bind developmental genes in fly embryos, with analogous co-occupancy of PRC1 and a Br140 ortholog, BRD1, at bivalent loci in human embryonic stem (ES) cells. We propose that identification of PRC1-Br140 "bivalent complexes" in fly embryos supports and extends the bivalency model posited in mammalian cells, in which the coexistence of H3K4me3 and H3K27me3 at developmental promoters represents a poised transcriptional state. We further speculate that local competition between acetylation and deacetylation may play a critical role in the resolution of bivalent protein complexes during development.
 
Overall design 36 samples in total:
human data set: 8 samples; HUES64 cells IP for BRD1 and RING1B
fly data set: 28 samples; Genomic binding/occupancy profiling of Fs(1)h, Br140, H3K27ac and H3K27me3 by high throughput sequencing
 
Contributor(s) Kuroda MI, Woolnough JL, Kang H
Citation(s) 29070704
Submission date Sep 20, 2017
Last update date May 15, 2019
Contact name Mitzi Kuroda
E-mail(s) mkuroda@genetics.med.harvard.edu
Organization name Brigham & Women's Hospital
Lab NRB168
Street address 77 Ave. Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (2)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (36)
GSM2797436 IP for BRD1 replicate 1
GSM2797437 IP for BRD1 replicate 2
GSM2797438 Input for BRD1 replicate 1
Relations
BioProject PRJNA408128
SRA SRP118320

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104059_RAW.tar 2.4 Gb (http)(custom) TAR (of BIGWIG, TDF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap