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Series GSE103987 Query DataSets for GSE103987
Status Public on Sep 09, 2023
Title MAP3K8 is a major regulator of inflammation in human airway epithelial cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this experiment we catalogue temporal variation in transcription occurring during IL1B stimulation under control (non-targeting siRNA control) conditions and partial MAP3K8 siRNA knockdown in the A549 cell line. We measure global gene transcription at 0, 1, 2, 4, 6, 8, and 12 hours using Affymetrix HuGene 1.1 ST array. Nineteen genes demonstrated a distinct temporal response to Il1B stimulation following suppression of MAP3K8.
 
Overall design A total of 48 RNA samples derived from the A549 cell line were analyzed as part of this study. The transcriptional response to IL1B stimulation (1 ng/ml IL-1β) was measured at 0, 1, 2, 4, 6, 8, and 12 hours under control and partial MAP3K8 knockdown conditions. A minimum of three replicates were used for each time point-treatment combination.
 
Contributor(s) Chiu C, Willis-Owen SA, Wong KC, Farrow SN, Zhang Y, Moffatt MF, Cookson WO
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Submission date Sep 19, 2017
Last update date Sep 09, 2023
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (48)
GSM2787373 A549-IL1B timecourse-MAP3K8 Control-8 hours-sample2
GSM2787374 A549-IL1B timecourse-MAP3K8 Knockdown-12 hours-sample2
GSM2787375 A549-IL1B timecourse-MAP3K8 Control-12 hours-sample3
Relations
BioProject PRJNA407890

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103987_RAW.tar 222.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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