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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 02, 2018 |
| Title |
Massively Parallel Single Nucleus Transcriptional Profiling Defines Spinal Cord Cell Types and Their Activity During Behavior |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the cellular basis of behavior, it is necessary to know the types of cells that exist in the nervous system and their contributions to function. Spinal cord networks are essential for sensory processing and motor behavior; however, the cellular correlates of these functions are not well understood. Here, we developed a massively parallel single nucleus RNA sequencing technique to first create a cell type atlas of the adult mouse spinal cord and then to use the transcriptional signatures of neuronal activity to map the cell types associated with specific behaviors. This approach, which we term Activity-SEQ, provided a broad and unbiased definition of the set of neuronal populations that were active in response to a painful stimulus and during locomotion. In the future, Activity-SEQ could provide a powerful strategy to reveal the molecular and cellular basis for a wide range of behaviors.
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| Overall design |
Single-cell RNA profiles for nuclei collected from 24 different mouse brains via Drop-Seq protocol. Included among the nuclei profiled were those from 9 control mice (f1, m1, M4, M5, F3, F4, facx, fbcx, Macx), 10 formalin-exposed mice, and 5 rotarod-challenged mice.
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| Contributor(s) |
Sathyamurthy A, Johnson KR, Li L, Matson KJ, Ryba AR, Bergman TB, Dobrott CI, Kelly MC, Kelley MW, Levine AJ |
| Citation(s) |
29466745 |
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| Submission date |
Sep 15, 2017 |
| Last update date |
Aug 13, 2019 |
| Contact name |
Kory R Johnson |
| E-mail(s) |
johnsonko@ninds.nih.gov
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| Phone |
301-402-1956
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| Organization name |
NINDS/NIH
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| Department |
DIR IT & Bioinformatics
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| Lab |
Bioinformatics Section
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| Street address |
10/3B01, 9000 Rockville Pike
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA407424 |
| SRA |
SRP117727 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103892_Expression_Count_Matrix.txt.gz |
23.8 Mb |
(ftp)(http) |
TXT |
| GSE103892_Sample_Cell_Cluster_Information.txt.gz |
111.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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