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Series GSE103453 Query DataSets for GSE103453
Status Public on Oct 01, 2017
Title Genome-wide analysis of SAGA subunit ada2b, and histones ubH2B, H2b, H3, H3K9ac, and H3K14ac in wt and Ataxin 7 mutants of 1-3 hr embryos of Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The Spt-Ada-Gcn5-acetyltransferase (SAGA) chromatin-modifying complex is a transcriptional coactivator that contains four different modules of subunits. The intact SAGA complex has been well characterized for its function in transcription regulation and development. However, little is known about the roles of individual modules within SAGA and if they have any SAGA independent functions. Here we demonstrate that the two enzymatic modules of Drosophila SAGA are differently required in oogenesis. Loss of the HAT activity blocks oogenesis, while loss of the DUB activity does not. However, the DUB module regulates a subset of genes in early embryogenesis and loss of the DUB subunits causes defects in embryogenesis. ChIP-seq analysis of ada2b, spt3, nonstop, and sgf11revealed that both the DUB and HAT modules bind most SAGA target genes even though many of these targets do not require the DUB module for expression. Furthermore, we found that the DUB module can bind to chromatin and regulate transcription independently of the rest of SAGA. Our results suggest that the DUB module has functions within SAGA as well as independent functions.
 
Overall design ChIP Seq analysis of 6 different chromatin associated factors in wt and Ataxin 7 mutant drosophila embryos was performed with 2 to 3 replicates of each factor.
 
Contributor(s) Li X, Seidel CW, Well LT, Workman JL, Abmayr SM
Citation(s) 30715476
Submission date Sep 05, 2017
Last update date May 15, 2019
Contact name Chris W Seidel
E-mail(s) seidel@phageT4.org
Phone 816 926 9054
Organization name Stowers Institute
Department Genomics
Lab Seidel
Street address 1000 E 50th St
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
 
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (33)
GSM2771622 H2B_ip_1749_1
GSM2771623 H2B_ip_1749_2
GSM2771624 H3_wt_ip_1
This SubSeries is part of SuperSeries:
GSE98865 Enzymatic modules of the SAGA chromatin-modifying complex play distinct roles in Drosophila gene expression and development
Relations
BioProject PRJNA401775
SRA SRP117058

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Supplementary file Size Download File type/resource
GSE103453_ada2b_1839_2.bed.gz 118.3 Kb (ftp)(http) BED
GSE103453_ada2b_1907_1.bed.gz 122.4 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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