|
| Status |
Public on Nov 20, 2017 |
| Title |
Single cell RNA-seq of ten-thousand epithelium cells from the mammary glands of mice in puberty, estrus and diestrus phases |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Epithelial cells were isolated by FACS from the mammary glands of pubescent (5 week old), estrus adult (10 week old) and diestrus adult (10 week old) female mice. Freshly sorted cells were submitted to a 10X Genomics Chromium System for single cell capture. cDNA synthesis and library preparation was done according to the protocol supplied by the manufacturer. Sequencing was carried out on an Illumina NextSeq500 sequencer using parameters recommended by 10X Genomics.
|
| |
|
| Overall design |
Transcriptional profiling was completed for 5387 total epithelial cells from pubescent (5 week old) mice, 2729 total epithelial cells from adult (10 week old) mice in the estrus phase, and 2439 total epithelial cells from adult (10 week old) mice in the diestrus phase.
|
| |
|
| Contributor(s) |
Smyth GK, Chen Y, Pal B, Visvader JE |
| Citation(s) |
29158510, 37994351 |
| |
| Submission date |
Aug 30, 2017 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE103275 |
Single cell RNA-seq of mouse mammary gland epithelium cells |
|
| Relations |
| BioProject |
PRJNA401672 |
| SRA |
SRP125700 |
Less...
More...