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Series GSE103224 Query DataSets for GSE103224
Status Public on Jul 02, 2018
Title Single-Cell Transcriptome Analysis of Lineage Diversity and Microenvironment in High-Grade Glioma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Despite extensive molecular characterization, we lack a comprehensive picture of lineage identity, differentiation, and microenvironmental composition in high-grade gliomas (HGGs). We sampled the cellular milieu of HGGs with massively-parallel single-cell RNA-Seq. While HGG cells can resemble glia or even immature neurons and form branched lineage structures, mesenchymal transformation results in unstructured populations. Glioma cells in a subset of mesenchymal tumors lose their neural lineage identity, express inflammatory genes, and co-exist with marked myeloid infiltration, implying a molecular interaction between glioma and immune cells. Finally, we found that myeloid cells can resemble microglia, macrophages, or a hybrid state, and enrichment of these cells is predictive of poor survival.
 
Overall design Performed single cell RNA-seq on tens of thousands of dissociated high-grade glioma tissue cells from 8 human patients.
 
Contributor(s) Yuan J, Sims PA, Levitin HM
Citation(s) 30041684
Submission date Aug 29, 2017
Last update date Jul 25, 2021
Contact name Peter Sims
Organization name Columbia University
Street address 3960 Broadway, 2nd Floor, Room 203AC
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM2758471 PJ016
GSM2758472 PJ017
GSM2758473 PJ018
Relations
BioProject PRJNA400576
SRA SRP116382

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103224_RAW.tar 52.5 Mb (http)(custom) TAR (of TXT)
GSE103224_README.txt 371 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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