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| Status |
Public on Jul 02, 2018 |
| Title |
Single-Cell Transcriptome Analysis of Lineage Diversity and Microenvironment in High-Grade Glioma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite extensive molecular characterization, we lack a comprehensive picture of lineage identity, differentiation, and microenvironmental composition in high-grade gliomas (HGGs). We sampled the cellular milieu of HGGs with massively-parallel single-cell RNA-Seq. While HGG cells can resemble glia or even immature neurons and form branched lineage structures, mesenchymal transformation results in unstructured populations. Glioma cells in a subset of mesenchymal tumors lose their neural lineage identity, express inflammatory genes, and co-exist with marked myeloid infiltration, implying a molecular interaction between glioma and immune cells. Finally, we found that myeloid cells can resemble microglia, macrophages, or a hybrid state, and enrichment of these cells is predictive of poor survival.
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| Overall design |
Performed single cell RNA-seq on tens of thousands of dissociated high-grade glioma tissue cells from 8 human patients.
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| Contributor(s) |
Yuan J, Sims PA, Levitin HM |
| Citation(s) |
30041684 |
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| Submission date |
Aug 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Peter Sims |
| Organization name |
Columbia University
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| Street address |
3960 Broadway, 2nd Floor, Room 203AC
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA400576 |
| SRA |
SRP116382 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE103224_RAW.tar |
52.5 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE103224_README.txt |
371 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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