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| Status |
Public on Oct 19, 2017 |
| Title |
Early-life gene expression in neurons modulates lasting epigenetic states. [Bisulfite-seq] |
| Organisms |
Mus musculus; Lambdavirus lambda |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
In mammals during the early postnatal period the environment plays a critical role in promoting the final steps in neuronal development. While epigenetic factors are thought to contribute to this process, the underlying molecular mechanisms remain poorly understood. Here we show that in the brain during early life the DNA methyltransferase DNMT3A transiently binds across transcribed regions of lowly expressed genes, and its binding specifies the pattern of DNA methylation at CA sequences (mCA) within these genes. We find that DNMT3A occupancy and mCA deposition within the transcribed regions of genes is negatively regulated by gene transcription and may be modified by early-life experience. Once deposited, mCA is bound by the methyl-DNA-binding protein MECP2 and functions in a rheostat-like manner to fine-tune the cell type-specific transcription of genes that are critical for brain function.
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| |
|
| Overall design |
Bisulfite-seq from mouse brain tissue and neurons
|
| |
|
| Contributor(s) |
Stroud H, Greenberg ME |
| Citation(s) |
29056337 |
| |
| Submission date |
Aug 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hume Stroud |
| Organization name |
UT Southwestern
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24075 |
Illumina NextSeq 500 (Escherichia virus Lambda) |
|
| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE103214 |
Early-life gene expression in neurons modulates lasting epigenetic states. |
|
| Relations |
| BioProject |
PRJNA400553 |
| SRA |
SRP116373 |
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