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| Status |
Public on Aug 30, 2018 |
| Title |
Identification of genes regulated by long noncoding RNA H19 in skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Soleus muscle has the most abundant H19 expression compared to other skeletal muscle tissues. In order to identify genes regulated by long noncoding RNA H19 in skeletal muscle, we performed RNA-Seq with dissected WT and H19KO soleus muscles from 21 week old mice. Among the differentially expressed genes, we found skeletal muscle - overexpressed gene DUSP27, which potentially plays an important role in regulating skeletal muscle glucose metabolisim by regulating the activitiy of AMPK, might be a target of H19 mediated regulation.
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| |
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| Overall design |
Soleus muscle was harvested from 21-week old mice, followed by total RNA extraction, library preparation and RNA-seq analysis to compare trancript profiles between WT and H19KO conditions.
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| Contributor(s) |
Zhang N, Carmichael GG, Huang Y |
| Citation(s) |
30201684 |
| |
| Submission date |
Aug 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Na Zhang |
| Organization name |
UConn Health
|
| Department |
Genetics and Genome Sciences
|
| Lab |
Gordon G. Carmichael
|
| Street address |
400 Farmington Ave
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA400485 |
| SRA |
SRP116339 |
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