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Series GSE103173 Query DataSets for GSE103173
Status Public on May 10, 2018
Title The transcription factor Bhlhe40 is a switch of inflammatory versus anti-inflammatory Th1 cell fate determination
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Type 1 T helper (Th1) cells play a critical role in host defense against intracellular pathogens and in autoimmune diseases by producing a key inflammatory cytokine interferon (IFN)-γ; some Th1 cells can also be anti-inflammatory through producing IL-10. However, the molecular switch for regulating the differentiation of inflammatory and anti-inflammatory Th1 cells is still elusive. Here we show that Bhlhe40-deficient CD4 Th1 cells produced less IFN-γ but substantially more IL-10 than wild type Th1 cells. Bhlhe40-mediated IFN-γ production was independent of transcription factor T-bet regulation. Mice with conditional deletion of Bhlhe40 in T cells succumbed to Toxoplasma gondii infection and blockage of IL-10 signaling during infection rescued these mice from death. Thus, our results demonstrate that transcription factor Bhlhe40 is a molecular switch for determining the fate of inflammatory and anti-inflammatory Th1 cells.
 
Overall design RNA-Seq analysis of gene expression in WT stimulated TH1 cells and in Bhlhe40 conditional KO stimulated TH1 mice.
 
Contributor(s) Fang Y, Suveena S, Dragana J, Rama Krishna G, Pan S, Gangqing H, Rao L, Sadiye R, Keji Z, Bing S, Jinfang Z
Citation(s) 29773643
Submission date Aug 28, 2017
Last update date May 15, 2019
Contact name Gangqing Hu
E-mail(s) michael.hu@hsc.wvu.edu
Organization name West Virginia University
Department MicroBiology, Immunology, and Cell Biology
Lab 2072A, HSC North, Floor 2
Street address 64 Medical Center Drive
City Morgantown
State/province West Virginia
ZIP/Postal code 26506-9177
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM2756565 GB1085
GSM2756566 GB1086
GSM2756567 GB1087
Relations
BioProject PRJNA400392
SRA SRP116313

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103173_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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