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Series GSE103023 Query DataSets for GSE103023
Status Public on Apr 06, 2018
Title Estrogen receptor ChIP-seq in response to mTOR inhibition
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have used ChIP-seq to profile binding of the estrogen receptor (ER) to chromatin in response to two mTOR inhibitors, i.e. everolimus (RAD001) and vistusertib (AZD2014). Two hours of treatment with these inhibitors significantly affected mTOR signaling, but surprisingly did not affect binding of ER to chromatin. This suggests that these mTOR inhibitors work through largely ER-independent mechanisms.
Overall design Estrogen receptor ChIP-seq was performed on chromatin from asynchronous MCF7 cells in full estrogenic media treated for 2 hours with vehicle, everolimus, or vistusertib.
Contributor(s) Michaloglou C, Crafter C, Siersbaek R, Delpuech O, Curwen J, Carnevalli L, Staniszewska A, Polanska U, Cheraghchi-Bashi A, Lawson M, Chernukhin I, McEwen R, Carroll J, Cosulich S
Citation(s) 29483206
Submission date Aug 24, 2017
Last update date May 15, 2019
Contact name Jason Carroll
Phone +44 1223 769649
Organization name Cancer Research UK, Cambridge Institute
Street address Li Ka Shing Centre, Robinson Way
City Cambridge
ZIP/Postal code CB2 ORE
Country United Kingdom
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (24)
GSM2752578 jc2906_MCF7_ER_Veh
GSM2752579 jc2907_MCF7_ER_RAD001
GSM2752580 jc2908_MCF7_ER_AZD2014
BioProject PRJNA399833
SRA SRP116074

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Supplementary file Size Download File type/resource
GSE103023_mcf7_er_azd2014_mtor_peaks.narrowPeak.gz 802.7 Kb (ftp)(http) NARROWPEAK
GSE103023_mcf7_er_rad001_mtor_peaks.narrowPeak.gz 1.1 Mb (ftp)(http) NARROWPEAK
GSE103023_mcf7_er_veh_mtor_peaks.narrowPeak.gz 947.6 Kb (ftp)(http) NARROWPEAK
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