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Series GSE103007 Query DataSets for GSE103007
Status Public on Dec 10, 2018
Title Variants of DNMT3A cause transcript-specific gene expression changes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The de novo DNA methyltransferase 3A (DNMT3A) plays a pivotal role in hematopoietic differentiation. In this study, we followed the hypothesis that alternative splicing of DNMT3A has characteristic epigenetic and functional sequels. Various transcripts of DNMT3A were either knocked down or overexpressed in human hematopoietic stem and progenitor cells resulting in complementary and transcript-specific DNA methylation (DNAm) and gene expression changes. Our results demonstrate that different splice variants of DNMT3A have distinct epigenetic and functional sequels.
 
Overall design Six biological replicas of cord blood-derived CD34+ hematopoietic stem and progenitor cells (HSPCs; #1-6) were isolated for the knockdown (KD; #1-3) and the overexpression (OE; #4-6) of DNMT3A transcripts. HSPCs were lentivirally infected with either shRNA or overexpression vectors 1 day after isolation. Control HSPCs were infected with a scrambled or empty vector. Genomic DNA was isolated at day 12 after infection, bisulfite converted and hybridized to the Illumina Infinium 450k Human Methylation Beadchip.
 
Contributor(s) Božić T, Frobel J, Raic A, Ticconi F, Kuo C, Heilmann-Heimbach S, Goecke TW, Zenke M, Jost E, Costa IG, Wagner W
Citation(s) 30582132
Submission date Aug 23, 2017
Last update date Dec 27, 2018
Contact name Wolfgang Wagner
E-mail(s) wwagner@ukaachen.de
Phone +49 241 8088611
Organization name RWTH Aachen University
Department Helmholtz Institute for Biomedical Engineering
Lab Stem Cell Biology and Cellular Engineering
Street address Pauwelsstrasse 20
City Aachen
ZIP/Postal code 52074
Country Germany
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (24)
GSM2752464 HSC_1 KD Tr.1+3
GSM2752465 HSC_1 KD Tr.2
GSM2752466 HSC_1 KD Tr.4
This SubSeries is part of SuperSeries:
GSE103008 Variants of DNMT3A cause transcript-specific DNA methylation and gene expression changes
Relations
BioProject PRJNA399751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE103007_RAW.tar 90.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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