NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE102940 Query DataSets for GSE102940
Status Public on Jan 11, 2018
Title Genome-wide maps of Super-enhancers in TH9 cells by H3K27Ac and BRD4 CHIP-Seq.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Th cell differentiation is transcriptionally regulated, relying on the induction of “lineage-defining” transcription factors. We report that formation of super-enhancers is critical in robust induction of Th9 cells and that assembly of the Il9 super-enhancers requires OX40-triggered chromatin H3K27 acetylation. Mechanistically, we found that OX40 costimulation induces RelB expression, which recruits the histone acetyltransferase p300 to the Il9 locus to catalyze H3K27 acetylation. This allows binding of the super-enhancer factor Brd4 to initiate assembly of the super-enhancer complex, which in turn drives robust Th9 induction. Thus, Th9 cells are induced in massive numbers upon OX40 costimulation and disruption of super-enhancers abolished Th9 induction in vitro and inhibited Th9-mediated allergic airway inflammation in vivo. Our data identify super-enhancers as a key mechanism of Th9 induction and uncover a new mechanism of Th cell differentiation.
 
Overall design Analysis of H3K27Ac histone modifications and BRD4 binding in CD4+ T cells cultured in vitro under Th9 differentiation conditions for 48 hours in the presence or absence of OX40 ligation.
 
Contributor(s) Li XC, Xiao X
Citation(s) 29339447
Submission date Aug 22, 2017
Last update date May 15, 2019
Contact name xiang xiao
E-mail(s) xxiao@houstonmethodist.org
Phone 7134418828
Organization name Houston Methodist Hospital and Houston Methodist Research Institute
Street address 6670 Bertner Avenue, R7-217
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (5)
GSM2750862 X1_H3K27Ac_ChIPSeq
GSM2750863 X2_H327Ac_ChIPSeq
GSM2750864 X1_BRD4_CHIPSeq
Relations
BioProject PRJNA399506
SRA SRP115941

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102940_RAW.tar 806.1 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap