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Series GSE102857 Query DataSets for GSE102857
Status Public on Oct 30, 2017
Title A molecular roadmap of the aorta-gonad-mesonephros region reveals BMPER as a novel regulator of HSC maturation [AGM]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the developing embryo, haematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA-sequencing over these spatio-temporal transitions in the AGM region, and supportive OP9 cell line.
 
Overall design RNA-sequencing profiles of the aorta-gonad-mesonephros region from E9.5 embryos and E10.5 embryos sub-dissected into dorsal (AoD), ventral (AoV) and urogenital ridges (UGR) and pooled from between 15 and 34 embryos in three separate experiments.
 
Contributor(s) Medvinsky A
Citation(s) 29093060
Submission date Aug 21, 2017
Last update date May 15, 2019
Contact name Alexander Medvinsky
Organization name University of Edinburgh
Department MRC Centre for Regenerative Medicine
Street address 5 Little France Drive
City Edinburgh
ZIP/Postal code EH16 4UU
Country United Kingdom
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (15)
GSM2747255 E9.5 AoD rep1
GSM2747256 E9.5 AoD rep2
GSM2747257 E9.5 AoD rep3
This SubSeries is part of SuperSeries:
GSE102859 A molecular roadmap of the aorta-gonad-mesonephros region reveals BMPER as a novel regulator of HSC maturation
Relations
BioProject PRJNA399129
SRA SRP115897

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102857_AGM_DESeq_RPM.csv.gz 1.8 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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