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Status |
Public on Jul 04, 2018 |
Title |
Eed, a member of the Polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We used R26R-tdTomato reporter mice activated by the Six2-TGC transgene to isolate cell populations co-expressing GFP and tdTomato by FACS from kidneys of control P0 mice. We observed two distinct populations of cells from control kidneys based on lower or higher tdTomato expression along with high GFP-expression from the Six2-TGC transgene. RNASeq experiment was conducted to analyze the transcriptome of these populations
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Overall design |
Examination of 2 different populations of cells derived from Six2-expressing kidney by RNA-seq.
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Contributor(s) |
Ettou S, Jain D, Kreidberg J |
Citation(s) |
29945864 |
Submission date |
Aug 15, 2017 |
Last update date |
Mar 21, 2019 |
Contact name |
Peter J Park |
E-mail(s) |
peter_park@harvard.edu
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Phone |
617-432-7373
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Organization name |
Harvard Medical School
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Department |
Center for Biomedical Informatics
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Street address |
10 Shattuck St
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA398390 |
SRA |
SRP115490 |