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| Status |
Public on Jun 25, 2019 |
| Title |
Identification of the coiled-coil domain as an essential element of MBD3 for preserving lineage commitment potential of ESCs |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
As one of approaches for elucidating the molecular bases which define the difference for counteracting defect of Mbd3-knockout ESCs between MBD3a mutant lacking MBD domain and that lacking coiled-coil domain, we explored the possibility that these two mutants are different in their ability to recruit PRC2 complex in close vicinity by ChIP-sequence analyses.
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| Overall design |
To inquire how SUZ12 binding genomic sites are globally affected dependently on the kinds of MBD3 mutants, ChIP-sequence analyses were conducted with antibody against SUZ12 using Mbd3-knockout ESCs which bear a MBD3a mutant lacking either MBD or coiled-coil domains.
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| Contributor(s) |
Hirasaki M, Kohda M, Okazaki Y, Okuda A |
| Citation(s) |
29761578 |
| |
| Submission date |
Aug 14, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Akihiko Okuda |
| E-mail(s) |
akiokuda@saitama-med.ac.jp
|
| Phone |
81-42-984-4787
|
| Organization name |
Saitama Medical University
|
| Department |
Research Center for Genomic Medicine
|
| Lab |
Division of Biomedical Sciences
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
3501241 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA398257 |
| SRA |
SRP115418 |
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