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Series GSE102593 Query DataSets for GSE102593
Status Public on Jan 24, 2018
Title Plasticity of life-long calorie restricted C57BL/6J mice in adapting to a medium-fat diet intervention at old age
Organism Mus musculus
Experiment type Expression profiling by array
Summary Calorie restriction (CR) is a dietary regimen that supports healthy aging. In this study we investigated the systemic and liver-specific responses caused by a diet switch to a medium-fat (MF) diet in 24-month-old life-long, CR-exposed mice. This study aimed to increase the knowledge base on dietary alterations of gerontological relevance. Nine-week-old C57BL/6J mice were exposed either to a control, CR or MF diet. At the age of 24 months, a subset of mice of the CR group was transferred to ad libitum MF feeding (CR-MF).The mice were sacrificed at the age of 28 months, then biochemical and molecular analyses were performed. Our results showed that, despite the long-term exposure to the CR regimen, mice in the CR-MF group displayed hyperphagia, rapid weight gain, and hepatic steatosis. However, no hepatic fibrosis/injury or alteration in CR-improved survival was observed in the diet switch group. The liver transcriptomic profile of CR-MF mice largely shifted to a profile similar to the MF-fed animals but leaving ~22% of the 1578 differentially regulated genes between the CR and MF diet groups comparable with the expression of the life-long CR group. Therefore, although the diet switch was performed at an old age, the CR-MF-exposed mice showed plasticity in coping with the challenge of a MF diet without developing severe liver pathologies.
Overall design Male C57BL/6J mice arrived at 7 weeks of age and allowed to acclimate for 2 weeks, receiving standard AIN-93G upon arrival. At the start of the diet intervention the mice were 9 weeks old and randomly distributed into three intervention groups: 1) Control diet receiving AIN-93W diet ad libitum; 2) CR diet receiving AIN-93W-CR in portions containing 70E% of the mean energy intake of the group of the control mice were provided each day at 15.30; 3) medium fat diet (MF) receiving AIN-93W-MF ad libitum. The long-term dietary invention was continued until a sacrifice at the age of 28 month, but in addition to the three diet groups, at 24 month the animals in the CR diet group were randomly assigned either to remain on the CR diet or undergo a diet switch to the MF diet (CR-MF). We performed various measurements on metabolic parameters and gene expression analysis. This entry represents the microarray data.
Contributor(s) Rusli F, Boekschoten MV, Borelli V, Sun C, Lute C, Menke AL, van den Heuvel J, Salvioli S, Franceschi C, Müller M, Steegenga WT
Citation(s) 29266667
Submission date Aug 12, 2017
Last update date Jan 24, 2018
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (27)
GSM2741506 Liver, AIN 93W (control) diet, age 28 months (G150_F04_099_A4_3)
GSM2741507 Liver, AIN 93W (control) diet, age 28 months (G150_F05_100_A4_5)
GSM2741508 Liver, AIN 93W (control) diet, age 28 months (G150_F06_102_A4_13)
BioProject PRJNA398117

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Supplementary file Size Download File type/resource
GSE102593_RAW.tar 122.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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