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Series GSE102460 Query DataSets for GSE102460
Status Public on Aug 29, 2017
Title BACH2 in human plasma cell fate decision
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We report here the broad transcriptomic program regulated by BACH2 transcription factor. We used a well suited in vitro model of B cell differentiation to evaluate transcriptomic program governed by BACH2 leading to Plasmocyte (PC) differentiation. In this model B cells were cultured with anti-BCR, CpG, CD40L and Interleukin-2 (IL2). This Interleukin triggers PC differentiation by directly repressing BACH2 expression. We artificially inhibit BACH2 expression by siRNA and found that this condition is sufficient to trigger PC differentiation without IL2. To understand global changes induced by enforced BACH2 downregulation we compared Chip-Sequencing data between activated B Cells and BACH2 deficient B cells (siBACH2). We found that BACH2 binds more than 3000 genes across the human genome. RNAsequencing comparing IL2 drivent committed cells and siBACH2 committed cells highlighs a large common trasncriptional program shared by both conditions and involved in B cell destiny. This study provides evidence that BACH2 is a guardian of B cell fate.
Overall design Differential gene expression data between 3 conditions (RNAsequencing in triplicate) : Uncomitted, comitted, and BACH2-deficient B cells integrated with BACH2 ChipSequencing on human activated B cells versus BACH2 deficient B cells as control.
Contributor(s) Hipp N, Delaloy C
Citation(s) 29129929
Submission date Aug 09, 2017
Last update date May 15, 2019
Contact name Nicolas HIPP
Organization name INSERM-
Lab U1236
Street address 2 Av. du Pr Léon Bernard
City Rennes
State/province France
ZIP/Postal code 35043 CEDEX
Country France
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM2738050 Day 4 CFSElowCD25hi population driven by IL2 priming rep 1
GSM2738051 Day 4 CFSElowCD25hi population driven by IL2 priming rep 2
GSM2738052 Day 4 CFSElowCD25hi population driven by IL2 priming rep 3
BioProject PRJNA397793
SRA SRP115151

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GSE102460_RAW.tar 468.4 Mb (http)(custom) TAR (of BW)
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Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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