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Series GSE101698 Query DataSets for GSE101698
Status Public on Feb 27, 2018
Title Evidence that DNA polymerase δ contributes to initiation of leading strand DNA replication in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Other
Summary To investigate nuclear DNA replication enzymology in vivo, we have studied Saccharomyces cerevisiae strains containing a pol2-16 mutation that inactivates the catalytic activities of DNA polymerase δ (Pol δ). Although pol2-16 mutants survive, their spore colonies are very tiny, with increased doubling time, larger than normal cells, aberrant nuclei, and rapid suppressor mutation accumulation. These phenotypes reveal a severe growth defect that is distinct from that of strains that lack Pol δ proofreading (pol2-4), consistent with the idea that Pol δ is the major leading strand replicase. Ribonucleotides are also incorporated into the pol2-16 genome in patterns consistent with leading strand replication by Pol δ when Pol δ is absent. More importantly, ribonucleotide distributions at replication origins suggest that in strains encoding all three replicases, Pol δ contributes to initiation of leading-strand replication. We describe two possible models.
Overall design Examination of location of ribonucleotides in multiple yeast strains bearing different DNA polymerase variants, either proficient or deficient in rnh201.
Contributor(s) Garbacz MA, Lujan SA, Burkholder AB, Cox PB, Wu Q, Zhou ZX, Haber JE, Kunkel TA
Citation(s) 29487291
Submission date Jul 20, 2017
Last update date May 15, 2019
Contact name Marta Anna Garbacz
Phone 9195410268
Organization name NIEHS/NIH
Department GISBL
Lab DNA Replication Fidelity Lab
Street address 111 T.W. alexander Dr.
City Research Triangle Park
State/province NC
ZIP/Postal code 27713
Country USA
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (28)
GSM2712335 TAK948_WT
GSM2712336 TAK949_pol2-16
GSM2712337 TAK951_pol1L868M
BioProject PRJNA395222
SRA SRP113233

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Supplementary file Size Download File type/resource
GSE101698_RAW.tar 396.0 Mb (http)(custom) TAR (of BW)
GSE101698_RNR1.fa.gz 3.5 Mb (ftp)(http) FA
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Raw data are available in SRA
Processed data provided as supplementary file

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