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| Status |
Public on Feb 05, 2008 |
| Title |
GATA-1-regulated erythroid microRNAs |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We employed a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. To search for GATA-1-regulated erythroid miRNAs, we used the Gata-1– erythroblast line G1E. These cells proliferate in culture as immature erythroid precursors and undergo terminal maturation when GATA-1 activity is restored. G1E-ER4 is a sub-line stably expressing an estrogen-activated form of GATA-1 (GATA-1 fused to the ligand binding domain of the estrogen receptor). Treatment of G1E-ER4 cells with estradiol induces a GATA-1-regulated program of gene expression with concomitant cellular maturation. We used a microarray to evaluate the expression of 292 different miRNAs in G1E-ER4 cells at 0 versus 24 hours after GATA-1 activation. Affymetrix gene expression profiling has previously been deposited (GEO accession no. GSE628).
Keywords: microRNA analysis of a cell-line model of erythroid maturation
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| Overall design |
Two condition experiment, 3 replicates each (independently grown and harvested) of untreated and estradiol-treated (24hrs) G1E-ER4 cells, which express an estrogen-responsive form of the GATA-1 transcription factor. Each sample is compared to a common reference sample, comprised of an equal mixture of all 6 experimental samples.
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| Contributor(s) |
Dore LC, Weiss MJ |
| Citation(s) |
18303114 |
| Submission date |
Jan 10, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Louis C Dore |
| E-mail(s) |
l-dore@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Feinberg School of Medicine
|
| Lab |
Division of Hematology/Oncology
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| Street address |
303 E Superior St., Lurie Bldg 5-250D
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL5531 |
Exiqon-Rat/Mouse/Human-540-v8.0 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA108681 |
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