|
| Status |
Public on Oct 01, 2017 |
| Title |
Sensors, Pathways and Transcription factors regulating IR-induced inflammatory transcriptional output [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Radiation induced interferon response is mediated by IRF1 but not by the IRF3. Phosphorylated p53 binds strongly within 1kb of TTS of p53 dependent genes that are potently induced by ionizing radiation.
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| |
|
| Overall design |
Bone marrow-derived macrophages were irradiated with 6Gy IR for 0, 0.5h, 1h, 2h and 6h. Chromatin from 0, 1h, 2h and 6h was immunoprecipitated with antibodies against IRF1 or IRF3. For p-p53 ChIP-seq, chromatin were prepared from 0, 0.5h, 1h, 2h and 6h time points.
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| |
|
| Contributor(s) |
Purbey PK, Smale ST |
| Citation(s) |
28930658 |
| |
| Submission date |
Jul 08, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Prabhat Kumar Purbey |
| E-mail(s) |
ppurbey@gmail.com
|
| Phone |
3102064946
|
| Organization name |
UCLA
|
| Department |
MIMG
|
| Lab |
Stephen T. Smale
|
| Street address |
675 Charles E. Young Drive South
|
| City |
los angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE100963 |
Sensors, Pathways and Transcription factors regulating IR-induced inflammatory transcriptional output |
|
| Relations |
| BioProject |
PRJNA393534 |
| SRA |
SRP111385 |
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