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| Status |
Public on Aug 09, 2017 |
| Title |
Gene expression profile in breast cancer cell lines using RNA sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to identify novel molecular targets associated with TNBC progression, we initially performed transcriptome analysis using RNA sequencing in breast cancer cell lines, classified as either the luminal subtype (MCF-7, T47D, ZR-75B) or basal-like subtype (MDA-MB-231, MDA-MB-435, Hs578T).
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| Overall design |
Total RNAs of each cell were isolated using the TRIzol reagent for RNA sequencing following manufacturer’s instructions. The total RNAs were treated with DNase I, purified with miRNeasy Mini Kit and subsequently quality checked using an Agilent 2100 Bioanalyzer. An Illumina platform was used to analyze transcriptomes with 90 bp paired-end library. Samples were paired-end sequenced with the Illumina HiSeq 2000 using HiSeq Sequencing kits.
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| Contributor(s) |
Kim S |
| Citation(s) |
31862882 |
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| Submission date |
Jul 06, 2017 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Seong-Jin Kim |
| E-mail(s) |
jasonsjkim@snu.ac.kr
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| Organization name |
Nano-Bio Medicine Research Center
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| Street address |
145 Gwanggyo-ro, Yeongtong-gu,
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| City |
Suwon-si, |
| ZIP/Postal code |
443-270 |
| Country |
South Korea |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA393329 |
| SRA |
SRP111199 |
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