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Series GSE100867 Query DataSets for GSE100867
Status Public on Jul 05, 2020
Title Association of microRNA-618 Expression With Altered Frequency and Activation of Plasmacytoid Dendritic Cells in Patients With Systemic Sclerosis
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To identify potential dysregulation of miRNA expression in plasmacytoid dendritic cells from Systemic Sclerosis patients (SSc), miRNA profiling was performed in pDCs from healthy donors or patients with the most severe fibrotic cutaneous form of SSc, namely in dcSSc, with either disease duration shorter (edcSSc) or longer than two years (ldcSSc).
Plasmacytoid dendritic cells (pDCs) are a critical source of type I interferons (IFNs) that can contribute to the onset and maintenance of autoimmunity. Molecular mechanisms leading to pDC dysregulation and persistent type I IFN signature are largely unexplored, especially in systemic sclerosis (SSc), a disease in which pDCs infiltrate fibrotic skin lesions and produce higher levels of IFNa as compared to healthy controls. To investigate potential microRNA–mediated epigenetic mechanisms underlying pDC dysregulation and type I IFN production in SSc. microRNA expression profiling and validation was performed in highly purified pDCs obtained from the peripheral blood of 3 independent cohorts of healthy controls and SSc patients. Possible functions of miR-618 on pDC biology were identified by overexpression in healthy pDCs. miR-618 expression was upregulated in pDCs of SSc patients, including those in the early stage of disease onset and not presenting with skin fibrosis. IRF8, a crucial transcription factor for pDC development and activation, was identified as a target of miR-618. miR-618 overexpression reduced the development of pDCs from CD34+ cells in-vitro and enhanced their ability to secrete IFNα, mimicking the pDC phenotype observed in SSc patients. miR-618 upregulation suppresses pDC development and increases their ability to secrete IFNα, potentially contributing to the type I IFN signature observed in SSc patients. Considering the importance of pDCs in the pathogenesis of SSc and other diseases characterized by a type I IFN signature, miR-618 potentially represents an important epigenetic target to regulate immune system homeostasis in these conditions.
 
Overall design BDCA-4+ pDC freshly isolated from pehripheral blood of 19 donors: 7 healthy (HC), 5 early diffuse cutaneous SSc patients (edcSSc) and 7 late diffuse cutaneous SSc patients (ldcSSc)
 
Contributor(s) Rossato M, Radstake TJ
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Submission date Jul 06, 2017
Last update date Jul 06, 2020
Contact name Marzia Rossato
E-mail(s) marzia.rossato@univr.it
Organization name University of Verona
Department Biotechnology
Lab CGF
Street address Strada Le Grazie 15
City Verona
ZIP/Postal code 37135
Country Italy
 
Platforms (1)
GPL8178 Illumina Human v1 MicroRNA expression beadchip
Samples (19)
GSM2695391 ExVivo_pDC_HC_1
GSM2695392 ExVivo_pDC_HC_2
GSM2695393 ExVivo_pDC_HC_3
Relations
BioProject PRJNA393316

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100867_RAW.tar 50.0 Kb (http)(custom) TAR
GSE100867_non-normalized.txt.gz 75.3 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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