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Series GSE100866 Query DataSets for GSE100866
Status Public on Jul 31, 2017
Title CITE-seq: Large scale simultaneous measuremnt of epitopes and transcriptomes in single cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.
 
Overall design Cord blood mononuclear cells were profiled by CITE-seq using a panel of 13 antibodies.
 
Contributor(s) Stoeckius M
Citation(s) 28759029
Submission date Jul 06, 2017
Last update date May 15, 2019
Contact name Marlon Stoeckius
E-mail(s) mstoeckius@nygenome.org
Organization name New York Genome Center
Department Innovation Lab
Street address 101 Avenue of the Americas
City New York
State/province New York
ZIP/Postal code 10013
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM2695379 ADT: CBMC_8K_13AB_10x
GSM2695380 RNA: CBMC_8K_13AB_10x
GSM2695381 ADT: PBMC_vs_flow_10x
Relations
BioProject PRJNA393315
SRA SRP111194

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100866_CBMC_8K_13AB_10X-ADT_clr-transformed.csv.gz 945.1 Kb (ftp)(http) CSV
GSE100866_CBMC_8K_13AB_10X-ADT_umi.csv.gz 189.8 Kb (ftp)(http) CSV
GSE100866_CBMC_8K_13AB_10X-RNA_umi.csv.gz 14.2 Mb (ftp)(http) CSV
GSE100866_CD8_merged-ADT_clr-transformed.csv.gz 58.4 Kb (ftp)(http) CSV
GSE100866_CD8_merged-ADT_umi.csv.gz 19.3 Kb (ftp)(http) CSV
GSE100866_CD8_merged-RNA_umi.csv.gz 1.4 Mb (ftp)(http) CSV
GSE100866_PBMC_vs_flow_10X-ADT_clr-transformed.csv.gz 466.9 Kb (ftp)(http) CSV
GSE100866_PBMC_vs_flow_10X-ADT_umi.csv.gz 129.4 Kb (ftp)(http) CSV
GSE100866_PBMC_vs_flow_10X-RNA_umi.csv.gz 10.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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