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| Status |
Public on Jan 25, 2018 |
| Title |
Distinct 5-methylcytosine profiles in poly(A)RNA from mouse bone marrow derived mast cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Purpose: To transcriptome widely reveal 5-mC regulation by Tet2, we performed bisulfite-sequencing of mRNAs from Tet2-deficient BMMCs and the control cells.Methods: Mouse BMMCs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse IL-3 and SCF. BMMCs were stained to confirm the surface expression of FcɛRI and c-Kit. Cells with purity >97.5% were used for RNA isolation. Results: We carried out an unbiased global analysis of m5C in poly(A)RNA of mouse bone marrow derived mast cells (BMMCs).We show that there were indeed much more mCs in Tet2-deficient group compared with the control, more than half of which located in genic region. Furthermore, we observed that although much less mCs distributed in the three elements of exon regions, more mCs located in 3`-UTR for Tet2-deficient group compared with the control.
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| Overall design |
RNA was isolated from Tet2 deficient bone marrow derived mast cells (BMMC) and control cells and RNA-Bisulfite sequencing was performed. We performed two biological replicates and each contain two technical replicates
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| Contributor(s) |
Shen Q, Cao X |
| Citation(s) |
29364877 |
| |
| Submission date |
Jul 03, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Qian Zhang |
| E-mail(s) |
oilpangpang@hotmail.com
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| Phone |
+8615921196443
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| Organization name |
Second military medical university
|
| Street address |
xiangyin road 800
|
| City |
shang hai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| GSM2692014 |
WT-biological replicate 1-technical replicate 1 |
| GSM2692015 |
WT-biological replicate 1-technical replicate 2 |
| GSM2692016 |
KO-biological replicate 1-technical replicate 1 |
| GSM2692017 |
KO-biological replicate 1-technical replicate 2 |
| GSM2692018 |
WT-biological replicate 2-technical replicate 1 |
| GSM2692019 |
WT-biological replicate 2-technical replicate 2 |
| GSM2692020 |
KO-biological replicate 2-technical replicate 1 |
| GSM2692021 |
KO-biological replicate 2-technical replicate 2 |
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| Relations |
| BioProject |
PRJNA392872 |
| SRA |
SRP110959 |
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