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Series GSE100508 Query DataSets for GSE100508
Status Public on Mar 05, 2019
Title The miRNA profiles of plasma extracellular vesicles (pEV) in melanoma patients and supernatant EV (sEV) of related cell cultures
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The dataset contains miRNA pEV profiles measured on Agilent Microarrays from three groups of melanoma patients (before or at resection, and after resection categorized into high-risk and low-risk groups) and healthy controls. These groups were compared to find differentially abundant miRNAs in the pEV of melanoma patients. In addition, two comparisons of short-term profile changes around the resection date are included, as well as six cell line sEV that play a role for the accompanying publication.
 
Overall design The four groups (14 healthy controls and 14 tumor, 15 high-risk, 11 low-risk patients) were subjected to differential expression analysis. The additional pEV samples served illustrational purposes as outgroup comparisons, and the six cell line samples were employed in an attempt to elucidate the putative source tissue of the pEV.
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There are two subsets: the first one consists of the above mentioned patient samples and was used for the differential expression analysis in melanoma context, which is why there are only 54 columns in the normalized matrix. The remainder of the samples were used in very specific comparative analyses with the groups from the first subset. These two approaches have completely different scopes and subgroup-dependent normalizations were performed as necessary.
 
Contributor(s) Koliha N, Dindorf J, Wild S, Baur AS, Eberhardt M
Citation(s) 30846484
Submission date Jun 26, 2017
Last update date Jul 25, 2021
Contact name Martin Eberhardt
Organization name University Hospital Erlangen
Department Dermatology
Street address Hartmannstr. 14
City Erlangen
ZIP/Postal code 91052
Country Germany
 
Platforms (1)
GPL22079 Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray (miRNA_107_Sep09)
Samples (68)
GSM2685625 pEV_healthy_rep1
GSM2685626 pEV_healthy_rep2
GSM2685627 pEV_healthy_rep3
Relations
BioProject PRJNA391975

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100508_RAW.tar 539.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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