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| Status |
Public on May 18, 2018 |
| Title |
Transcriptional Profiling Reveals Extraordinary Diversity Among Skeletal Muscle Tissues |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscles are a diverse family of highly specialized tissues that perform a wide array of physiological activities and maintain whole-body glucose metabolism and energy homeostasis. Functional diversity is a distinctive feature of muscle tissues, which demonstrate remarkable variability in their speed of contraction, metabolic profile, resistance to fatigue, and regenerative capacity. Unsurprisingly, many disorders of skeletal muscle afflict a remarkably specific subset of tissues, including muscular dystrophies, cancer cachexia, aging sarcopenia, and amyotrophic lateral sclerosis. Taken as a whole, these observations indicate that specific genetic programs establish and maintain physiological specialization of muscle tissues. Elucidating these genetic programs is essential to understanding muscle specialization and propensity for disease. Nevertheless, most global profiling studies performed to-date have not directly addressed intrinsic variability between different muscle tissues. This gap in the literature is particularly glaring for more sophisticated mechanisms of gene expression regulation such as circadian control, post-transcriptional regulation and non-coding RNA expression, despite their well-established roles in many disease mechanisms. In an effort to understand the variability present within specific skeletal muscles with respect to gene expression, our lab has performed RNA-Seq on a variety of skeletal muscle tissues.
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| Overall design |
126 samples, 17 mouse tissues (all from males), 2 female rat tissues, 2 male rat tissues. Six replicates for each tissue; each replicate is 3 individual samples pooled. For mouse tissues, 3 are smooth muscle, 3 are cardiac muscle and 11 are skeletal muscle. For male & female rat samples, both tissues are skeletal.
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| Contributor(s) |
Terry EE, Zhang X, Hoffmann C, Hughes LD, Lewis S, Li J, Riley L, Lahens N, Gong M, Andrade F, Esser KA, Hughes ME |
| Citation(s) |
29809149 |
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| Submission date |
Jun 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michael Hughes |
| E-mail(s) |
michael.evan.hughes@gmail.com
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| Organization name |
Washington University
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| Department |
Internal Medicine
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| Lab |
Hughes Lab
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| Street address |
425 South Euclid Avenue
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| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (126)
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| Relations |
| BioProject |
PRJNA391968 |
| SRA |
SRP110541 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100505_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of BED, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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