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Series GSE100393 Query DataSets for GSE100393
Status Public on Jun 23, 2017
Title Transcriptional profiling of small intestinal lamina propria Dendritic cells by microarray
Organism Mus musculus
Experiment type Expression profiling by array
Summary CD103+CD11b+ dendritic cells (DC) are unique to the intestine, but the factors governing their differentiation are unclear. Here we show that transforming growth factor receptor 1 (TGF beta 1) has an indispensable, cell intrinsic role in the development of these cells. Deletion of Tgfbr1 results in markedly fewer intestinal CD103+CD11b+ DCs and a reciprocal increase in the CD103–CD11b+ DC subset. Transcriptional profiling identifies markers that define the CD103+CD11b+ DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103+CD11b+ DCs in CD11c-Cre.Tgfbr1fl/fl mice reflects defective differentiation from CD103–CD11b+ intermediaries, rather than an isolated loss of CD103 expression. The defect in CD103+CD11b+ DCs is accompanied by reduced generation of antigen-specific, inducible FoxP3+ regulatory T (Treg) cells in vitro and in vivo, and by reduced numbers of endogenous TH17 cells in the intestinal mucosa. Thus, TGF beta 1 mediated signalling may explain the tissue-specific development of these unique DCs.
Overall design Dendritic cell and Macrophage subpopoulations were isolated from the Small Intestine of Healthy Steady State mice, RNA was isolated and sent for microarray analysis
Contributor(s) Bain CC, Montgomery J, Scott CL, Kel JM, Girard-Madoux MJ, Martens L, Zangerle-Murray TF, Ober-Blöbaum J, Lindenbergh-Kortleve D, Samsom JN, Henri S, Lawrence T, Saeys Y, Malissen B, Dalod M, Clausen BE, Mowat AM
Citation(s) 28931816
Submission date Jun 22, 2017
Last update date Jul 25, 2021
Contact name Liesbet Martens
Organization name VIB-University of Ghent
Department VIB Inflammation Research Center
Street address Technologiepark 927
City Zwijnaarde
ZIP/Postal code 9052
Country Belgium
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (15)
GSM2680230 DC_CD64-CD103+CD11b+_rep1
GSM2680231 DC_CD64-CD103+CD11b+_rep2
GSM2680232 DC_CD64-CD103+CD11b+_rep3
BioProject PRJNA391507

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GSE100393_RAW.tar 58.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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