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| Status |
Public on Jun 23, 2017 |
| Title |
Transcriptional profiling of small intestinal lamina propria Dendritic cells by microarray |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD103+CD11b+ dendritic cells (DC) are unique to the intestine, but the factors governing their differentiation are unclear. Here we show that transforming growth factor receptor 1 (TGF beta 1) has an indispensable, cell intrinsic role in the development of these cells. Deletion of Tgfbr1 results in markedly fewer intestinal CD103+CD11b+ DCs and a reciprocal increase in the CD103–CD11b+ DC subset. Transcriptional profiling identifies markers that define the CD103+CD11b+ DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103+CD11b+ DCs in CD11c-Cre.Tgfbr1fl/fl mice reflects defective differentiation from CD103–CD11b+ intermediaries, rather than an isolated loss of CD103 expression. The defect in CD103+CD11b+ DCs is accompanied by reduced generation of antigen-specific, inducible FoxP3+ regulatory T (Treg) cells in vitro and in vivo, and by reduced numbers of endogenous TH17 cells in the intestinal mucosa. Thus, TGF beta 1 mediated signalling may explain the tissue-specific development of these unique DCs.
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| Overall design |
Dendritic cell and Macrophage subpopoulations were isolated from the Small Intestine of Healthy Steady State mice, RNA was isolated and sent for microarray analysis
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| Contributor(s) |
Bain CC, Montgomery J, Scott CL, Kel JM, Girard-Madoux MJ, Martens L, Zangerle-Murray TF, Ober-Blöbaum J, Lindenbergh-Kortleve D, Samsom JN, Henri S, Lawrence T, Saeys Y, Malissen B, Dalod M, Clausen BE, Mowat AM |
| Citation(s) |
28931816 |
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| Submission date |
Jun 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Liesbet Martens |
| E-mail(s) |
liesbet.martens@irc.vib-ugent.be
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| Organization name |
VIB-University of Ghent
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| Department |
VIB Inflammation Research Center
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| Street address |
Technologiepark 927
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| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA391507 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100393_RAW.tar |
58.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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