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| Status |
Public on Jun 19, 2019 |
| Title |
Whole transcriptome sequencing of the human thyroid primary cells with knock-down of the NRG1 gene |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The protein encoded by the NRG1 gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Here we report gene expression profiles of human thyroid primary cells on Day 5 treated with NRG1 siRNA. The primary cells were generated from fresh nontumorous thyroid tissues obtained from thyroid cancer patients. A number of new dysregulated genes of NRG1 were discovered. This study reveals a novel role of NRG1 in downstream gene regulation and provides a new explanation of NRG1’s function in thyroid cancer.
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| Overall design |
Profiling of 3 human thyroid primary cells (generated from 3 different patient samples) transfected with NRG1 siRNA (75pmol) was performed using RNA-seq. Cells were cultured for 24h after transfection and then lysed prior to RNA isolation, DNase treatment, purification and RNA-seq library construction.
Profiling of 3 human thyroid primary cultures (3 nontarget siRNA treated) derived from the fresh thyroid tissues of 3 individuals was also performed using RNA-seq.
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| Contributor(s) |
de la Chapelle A, Wang Y, He H |
| Citation(s) |
29121253 |
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| Submission date |
Jun 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yanqiang Wang |
| Organization name |
The Ohio State University
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| Street address |
12th w street
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| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA391313 |
| SRA |
SRP110035 |
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