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| Status |
Public on May 02, 2018 |
| Title |
Single-Cell Transcriptomics and Fate Mapping of Ependymal Cells Reveals an Absence of Neural Stem Cell Function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ependymal cells are multi-ciliated cells that form the brain’s ventricular epithelium and a niche for neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ). In addition, ependymal cells are suggested to be latent NSCs with a capacity to acquire neurogenic function. This remains highly controversial due to a lack of prospective in vivo labeling techniques that can effectively distinguish ependymal cells from neighboring V-SVZ NSCs. We describe a transgenic system that allows for targeted labeling of ependymal cells within the V-SVZ. Single-cell RNA-seq revealed that ependymal cells are enriched for cilia-related genes and share several stem-cell-associated genes with neural stem or progenitors. Under in vivo and in vitro neural-stem- or progenitor-stimulating environments, ependymal cells failed to demonstrate any suggestion of latent neural-stem-cell function. These findings suggest remarkable stability of ependymal cell function and provide fundamental insights into the molecular signature of the V-SVZ niche.
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| Overall design |
We FACS collected three samples of cells from the dissociated subventricular zone of aSMACreERT2::tdTomato/Sox2-GFP mice. These included one sample of tdTomato/GFP double positive cells, and two samples of tdTomato-negative/GFP-positive cells. 10X Chromium technology was used to prepare single cell barcoded RNA sequencing libraries from each sample. These samples were subsequently sequenced on the Illumina HiSeq 2500 to a total average depth of approx. 50 million reads/sample.
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| Contributor(s) |
Shah PT, Stratton JA, Biernaskie J |
| Citation(s) |
29727663, 34335193 |
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| Submission date |
Jun 21, 2017 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Jeff Biernaskie |
| E-mail(s) |
jeff.biernaskie@ucalgary.ca
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| Organization name |
University of Calgary - Faculty of Veterinary Medicine
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| Street address |
3300 Hospital Dr NW
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| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA391311 |
| SRA |
SRP110034 |
