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| Status |
Public on Sep 09, 2020 |
| Title |
Cytosine methylation dynamics during spermatogenesis and post-testicular sperm maturation |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We carried out whole genome bisulfite sequencing in seven purified sperm populations, including primary spermatocytes, early and late round spermatids, and mature sperm isolated from the caput, corpus, and cauda epididiymis, and from the vas deferens.
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| Overall design |
Testicular sperm populations were isolated by elutriation. Epididymal and vas deferens sperm were obtained by gently squeezing sperm out of the dissected tissue. Sperm were washed several times prior to genomic DNA extraction, and purified gDNA was subject to WGBS.
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| Contributor(s) |
Rando OJ, Galan C, Serra R, Krueger F |
| Citation missing |
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| |
| Submission date |
Jun 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Felix Krueger |
| E-mail(s) |
fkrueger@altoslabs.com
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| Organization name |
Altos Labs
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| Department |
Bioinformatics
|
| Street address |
Granta Park
|
| City |
Cambridge |
| ZIP/Postal code |
CB21 6GP |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA391076 |
| SRA |
SRP109758 |
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