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| Status |
Public on Jun 20, 2017 |
| Title |
Expression analysis of Arabidopsis thaliana Col-0 and parg1 with and without PARP inhibitor (3AB, 3MB), with and without MAMP (flg22, elf18) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Genome-wide gene expression analysis of the effects of PARP inhibitor (3AB) and, separately, a parg1 knockout, on early microbe-associate molecular pattern (MAMP)-induced gene expression in the plant basal defense response. Arabidopsis thaliana wild-type (Col-0), 3AB-treated and parg1-2 T-DNA knockout plants responding to the MAMP elicitors flg22 or elf18 were studied.
The mutant and PARP inhibitors analyzed in this study are further described in Adams-Phillips, L.,C., Briggs, A.,G., Bent, A., F. 2010. Disruption of poly(ADP-ribosyl)ation mechanisms alters responses of Arabidopsis to biotic stress. Planty Physiol. 152(1):267-80 DOI: 10.1104/pp.109.148049.
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| Overall design |
A 27 chip study using total RNA recovered from three separate wild-type extracts of Arabidopsis Col-0 and three separate cultures of a parg1-2 knockout strain, in which a TDNA insertion has knocked out expression of At2g31870, treated for 1 hour with vehicle, flg22, elf28, 3AB, or 3MB. Each chip measures the expression level of 30,387 genes from Arabidopsis thaliana Col-0 with 6 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy and three-fold biological redundancy.
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| Contributor(s) |
Bent AF, Briggs AG, Adams-Phillips LC |
| Citation(s) |
29284022 |
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| Submission date |
Jun 19, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Amy Graham Briggs |
| E-mail(s) |
briggsa@beloit.edu
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| Phone |
6083632349
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| Organization name |
Beloit College
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| Department |
Biology
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| Street address |
700 College St.
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| City |
Beloit |
| State/province |
WI |
| ZIP/Postal code |
53511 |
| Country |
USA |
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| Platforms (1) |
| GPL23603 |
NimbleGen Arabidopsis thaliana expression array [2006-08-28_Arabidopsis_60mer_expr] |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA390984 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100205_2006-08-28_Arabidopsis_60mer_expr_RMA.calls.xlsx |
20.6 Mb |
(ftp)(http) |
XLSX |
| GSE100205_RAW.tar |
230.7 Mb |
(http)(custom) |
TAR (of CALLS, PAIR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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