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Status
Public on Jan 06, 2010
Title
MIT-HRH-Celegans-MAS8
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Caenorhabditis elegans Manufacturer
Whitehead Institute
Manufacture protocol
Microarray probes were oligonucleotides with sequences complementary to microRNAs and other small RNAs. Each probe was modified with a free amino group linked to its 5' terminus through a 6-carbon spacer (IDT) and was printed onto amine-binding slides (CodeLink, Amersham Biosciences). Printing and hybridization were done using the protocols from the slide manufacturer with the following modifications: the oligonucleotide concentration for printing was 20 μM in 150 mM sodium phosphate pH 8.5, and hybridization was at 50°C for 6 h.
Submission date
Dec 18, 2009
Last update date
Jan 06, 2010
Contact name
Ezequiel Alvarez-Saavedra
Organization name
MIT
Department
Biology
Lab
Horvitz
Street address
77 Massachusetts Avenue Room 68-411
City
Cambridge
State/province
MA
ZIP/Postal code
02139
Country
USA
Samples (6)
GSM487566 , GSM487567 , GSM487568 , GSM487569 , GSM487570 , GSM487571 Series (1)
GSE19557 miRNA expression in C. elegans fem-2(b245ts) and fem-3(q20ts) mutants
Data table header descriptions ID Name oligo name
small RNA small RNA detected
Position position on the array. Numbers correspond to block-row-column.
SEQUENCE spotted oligonucleotide sequence
miRNA_ID SPOT_ID
Data table ID Name small RNA Position SEQUENCE miRNA_ID SPOT_ID 1
emc13
miR-42
1-1-1
CTGTAGATGTTAACCCGGTG
miR-42
2
emc13
miR-42
1-1-2
CTGTAGATGTTAACCCGGTG
miR-42
3
emc13
miR-42
1-1-3
CTGTAGATGTTAACCCGGTG
miR-42
4
emc13
miR-42
1-1-4
CTGTAGATGTTAACCCGGTG
miR-42
5
emc14
miR-43
1-1-5
GCGACAGCAAGTAAACTGTG
miR-43
6
emc14
miR-43
1-1-6
GCGACAGCAAGTAAACTGTG
miR-43
7
emc14
miR-43
1-1-7
GCGACAGCAAGTAAACTGTG
miR-43
8
emc14
miR-43
1-1-8
GCGACAGCAAGTAAACTGTG
miR-43
9
emc15
miR-44
1-1-9
AGCTGAATGTGTCTCTAGTC
miR-44
10
emc15
miR-44
1-1-10
AGCTGAATGTGTCTCTAGTC
miR-44
11
emc15
miR-44
1-1-11
AGCTGAATGTGTCTCTAGTC
miR-44
12
emc15
miR-44
1-1-12
AGCTGAATGTGTCTCTAGTC
miR-44
13
emc16
miR-45
1-2-1
AGCTGAATGTGTCTCTAGTC
miR-45
14
emc16
miR-45
1-2-2
AGCTGAATGTGTCTCTAGTC
miR-45
15
emc16
miR-45
1-2-3
AGCTGAATGTGTCTCTAGTC
miR-45
16
emc16
miR-45
1-2-4
AGCTGAATGTGTCTCTAGTC
miR-45
17
emc17
miR-46
1-2-5
TGAAGAGAGCGACTCCATGA
miR-46
18
emc17
miR-46
1-2-6
TGAAGAGAGCGACTCCATGA
miR-46
19
emc17
miR-46
1-2-7
TGAAGAGAGCGACTCCATGA
miR-46
20
emc17
miR-46
1-2-8
TGAAGAGAGCGACTCCATGA
miR-46
576 31 Kbytes .Supplementary data files not provided
