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Platform GPL9815 Query DataSets for GPL9815
Status Public on Jan 06, 2010
Title MIT-HRH-Celegans-MAS8
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Caenorhabditis elegans
Manufacturer Whitehead Institute
Manufacture protocol Microarray probes were oligonucleotides with sequences complementary to microRNAs and other small RNAs. Each probe was modified with a free amino group linked to its 5' terminus through a 6-carbon spacer (IDT) and was printed onto amine-binding slides (CodeLink, Amersham Biosciences). Printing and hybridization were done using the protocols from the slide manufacturer with the following modifications: the oligonucleotide concentration for printing was 20 μM in 150 mM sodium phosphate pH 8.5, and hybridization was at 50°C for 6 h.
Submission date Dec 18, 2009
Last update date Jan 06, 2010
Contact name Ezequiel Alvarez-Saavedra
Organization name MIT
Department Biology
Lab Horvitz
Street address 77 Massachusetts Avenue Room 68-411
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Samples (6) GSM487566, GSM487567, GSM487568, GSM487569, GSM487570, GSM487571
Series (1)
GSE19557 miRNA expression in C. elegans fem-2(b245ts) and fem-3(q20ts) mutants

Data table header descriptions
Name oligo name
small RNA small RNA detected
Position position on the array. Numbers correspond to block-row-column.
SEQUENCE spotted oligonucleotide sequence

Data table
ID Name small RNA Position SEQUENCE miRNA_ID SPOT_ID
1 emc13 miR-42 1-1-1 CTGTAGATGTTAACCCGGTG miR-42
2 emc13 miR-42 1-1-2 CTGTAGATGTTAACCCGGTG miR-42
3 emc13 miR-42 1-1-3 CTGTAGATGTTAACCCGGTG miR-42
4 emc13 miR-42 1-1-4 CTGTAGATGTTAACCCGGTG miR-42
5 emc14 miR-43 1-1-5 GCGACAGCAAGTAAACTGTG miR-43
6 emc14 miR-43 1-1-6 GCGACAGCAAGTAAACTGTG miR-43
7 emc14 miR-43 1-1-7 GCGACAGCAAGTAAACTGTG miR-43
8 emc14 miR-43 1-1-8 GCGACAGCAAGTAAACTGTG miR-43
9 emc15 miR-44 1-1-9 AGCTGAATGTGTCTCTAGTC miR-44
10 emc15 miR-44 1-1-10 AGCTGAATGTGTCTCTAGTC miR-44
11 emc15 miR-44 1-1-11 AGCTGAATGTGTCTCTAGTC miR-44
12 emc15 miR-44 1-1-12 AGCTGAATGTGTCTCTAGTC miR-44
13 emc16 miR-45 1-2-1 AGCTGAATGTGTCTCTAGTC miR-45
14 emc16 miR-45 1-2-2 AGCTGAATGTGTCTCTAGTC miR-45
15 emc16 miR-45 1-2-3 AGCTGAATGTGTCTCTAGTC miR-45
16 emc16 miR-45 1-2-4 AGCTGAATGTGTCTCTAGTC miR-45
17 emc17 miR-46 1-2-5 TGAAGAGAGCGACTCCATGA miR-46
18 emc17 miR-46 1-2-6 TGAAGAGAGCGACTCCATGA miR-46
19 emc17 miR-46 1-2-7 TGAAGAGAGCGACTCCATGA miR-46
20 emc17 miR-46 1-2-8 TGAAGAGAGCGACTCCATGA miR-46

Total number of rows: 576

Table truncated, full table size 31 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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