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Platform GPL9173 Query DataSets for GPL9173
Status Public on Sep 10, 2010
Title Ambion mirVana™ miRNA Probe Set 1564V2 Oct06_IZKF (condensed)
Technology type spotted oligonucleotide
Distribution custom-commercial
Organisms Homo sapiens; Mus musculus; Rattus norvegicus
Manufacturer Laboratory for microarray applications, IZKF, University of Wuerzburg
Manufacture protocol 1. Oligos are arrayed in 96 well plates. Each well contains 500 pmol of 42–46 nucleotides (nt) long oligos and consist of an 18–24 nt segment that targets a specific known human, mouse, or rat miRNA, plus spacer and attachment sequences for coupling to the surface of the microarray slide
2. Add 3 x SSC + 1.5 M Betaine (in Rnase-free water) to 10 uM
3. Seal plates and wrap them in saran wrap
4. Let it resuspend in a humid chamber on a shaker (60 rpm) over night
5. Centrifuge plates at 2000 rpm for 3 minutes.
6. Rearray 10 µL into 384-well, V-bottom plates
7. Centrifuge plates at 2000 rpm for 3 minutes.
8. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 15 minutes before next cassette is needed to begin centrifugation
9. After the print run is completed, allow plates to dry through passive evaporation in a protected environment
10. Spots are printed in quadruplicates
11. For each subsequent preparation of these plates for a print run, add water to the wells instead of print buffer. The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary during each dip
Support glass
Coating other
 
Description support = Nexterion® Slide E, coating = epoxy

The Platform data table reflects a condensed representation of the array's quadruplicate features. The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.
 
Contributor(s) Kneitz S
Submission date Sep 10, 2009
Last update date Sep 10, 2010
Contact name Burkhard Gerhard Kneitz
E-mail(s) Kneitz_B@klinik.uni-wuerzburg.de
Phone +49-931-201-32700
Organization name University of Wuerzburg
Department Klinik und Poliklinik für Urologie und Kinderurologie
Street address Oberdürrbacher Str. 6
City Wuerzburg
ZIP/Postal code 97080
Country Germany
 
Samples (20) GSM463747, GSM463748, GSM463749, GSM463750, GSM463751, GSM463752 
Series (1)
GSE18671 miRNA expression data from human high and low risk prostate carcinoma

Data table header descriptions
ID
Probe Name microRNA name
Human Mature Sanger Registry Name
Human Sanger Accession Number(s)
Mouse Mature Sanger Registry Name
Mouse Sanger Accession Number(s)
Rat Mature Sanger Registry Name
Rat Sanger Accession Number(s)
miRNA_LIST Sanger miRNA identifier
SEQUENCE
SPOT_ID spot identifier

Data table
ID Probe Name Human Mature Sanger Registry Name Human Sanger Accession Number(s) Mouse Mature Sanger Registry Name Mouse Sanger Accession Number(s) Rat Mature Sanger Registry Name Rat Sanger Accession Number(s) miRNA_LIST SEQUENCE SPOT_ID
1 hsa_miR_296 hsa-miR-296 MIMAT0000690 mmu-miR-296 MIMAT0000374 rno-miR-296 MIMAT0000898 hsa-miR-296, mmu-miR-296, rno-miR-296 AGGGCCCCCCCUCAAUCCUGU
2 rno_miR_350 rno-miR-350 MIMAT0000604 rno-miR-350 UUCACAAAGCCCAUACACUUUCAC
3 hsa_miR_519d hsa-miR-519d MIMAT0002853 hsa-miR-519d CAAAGUGCCUCCCUUUAGAGUGU
4 hsa_miR_493_5p hsa-miR-493-5p MIMAT0002813 hsa-miR-493-5p UUGUACAUGGUAGGCUUUCAUU
9 rno_miR_349 rno-miR-349 MIMAT0000599 rno-miR-349 CAGCCCUGCUGUCUUAACCUCU
10 hsa_miR_484 hsa-miR-484 MIMAT0002174 mmu-miR-484 MIMAT0003127 hsa-miR-484, mmu-miR-484 UCAGGCUCAGUCCCCUCCCGAU
11 mmu_miR_99a mmu-miR-99a MIMAT0000131 mmu-miR-99a ACCCGUAGAUCCGAUCUUGU
12 hsa_miR_515_5p hsa-miR-515-5p MIMAT0002826 hsa-miR-515-5p UUCUCCAAAAGAAAGCACUUUCUG
17 hsa_miR_337 hsa-miR-337 MIMAT0000754 hsa-miR-337 UCCAGCUCCUAUAUGAUGCCUUU
18 hsa_miR_128b hsa-miR-128b MIMAT0000676 mmu-miR-128b MIMAT0000675 rno-miR-128b MIMAT0000835 hsa-miR-128b, mmu-miR-128b, rno-miR-128b UCACAGUGAACCGGUCUCUUUC
19 hsa_miR_509 hsa-miR-509 MIMAT0002881 hsa-miR-509 UGAUUGGUACGUCUGUGGGUAGA
20 mmu_miR_325 mmu-miR-325 MIMAT0000558 rno-miR-325 MIMAT0000557 mmu-miR-325, rno-miR-325 CCUAGUAGGUGCUCAGUAAGUGU
25 hsa_miR_96 hsa-miR-96 MIMAT0000095 hsa-miR-96 UUUGGCACUAGCACAUUUUUGC
26 mmu_miR_30e_AS mmu-miR-30e* MIMAT0000249 mmu-miR-30e* CUUUCAGUCGGAUGUUUACAG
27 hsa_miR_125b hsa-miR-125b MIMAT0000423 mmu-miR-125b MIMAT0000136 rno-miR-125b MIMAT0000830 hsa-miR-125b, mmu-miR-125b, rno-miR-125b UCCCUGAGACCCUAACUUGUGA
28 mmu_miR_211 mmu-miR-211 MIMAT0000668 rno-miR-211 MIMAT0000882 mmu-miR-211, rno-miR-211 UUCCCUUUGUCAUCCUUUGCCU
65 hsa_miR_455 hsa-miR-455 MIMAT0003150 hsa-miR-455 UAUGUGCCUUUGGACUACAUCG
66 hsa_miR_101 hsa-miR-101 MIMAT0000099 mmu-miR-101a MIMAT0000133 rno-miR-101a MIMAT0000823 hsa-miR-101, mmu-miR-101a, rno-miR-101a UACAGUACUGUGAUAACUGAAG
67 hsa_miR_486 hsa-miR-486 MIMAT0002177 mmu-miR-486 MIMAT0003130 hsa-miR-486, mmu-miR-486 UCCUGUACUGAGCUGCCCCGAG
68 hsa_miR_520d_AS hsa-miR-520d* MIMAT0002855 hsa-miR-520d* UCUACAAAGGGAAGCCCUUUCUG

Total number of rows: 665

Table truncated, full table size 61 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary file Size Download File type/resource
GPL9173_full_array_layout.txt.gz 31.7 Kb (ftp)(http) TXT

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