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Status
Public on Oct 27, 2009
Title
miRNA microarrays (676)
Technology type
spotted DNA/cDNA
Distribution
commercial
Organisms
Homo sapiens ; Mus musculus ; Rattus norvegicus Manufacturer
Miltenyi Biotec GmbH, Cologne, Germany
Manufacture protocol
1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted four times on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides using a customized piezoelectric spotter.
Description
Each microarray contained a total of 256 DNA oligonucleotides with a sequence being reverse complement to the respective mature miRNA or control RNA, representing a total of 22 miRNA sequences (standard), 29 different variants of oligonucleoties and 13 controls. All probes were spotted in quadruplicates. The 22 miRNA sequences covered sequence verified miRNAs as deposited in the miRBase sequence database version 10.0. The 29 different variant oligonucleotides were chosen as follows: 9 different oligonucleotide variants for miR-122a and miR-16, including probes with (a) spacer sequences (6, 12 or 18 nucleotides in length named SP6, SP12 and SP18); (b) variants with omitted linker named NO-LINK; (c) dimer (DI) and trimer (TRI) variants and (d) oligo variants with adjusted TM (MELT). Oligo variants with adjusted TM were added for seven further miRNAs besides miR-16 and miR-122a. The 13 controls presented 10 positive and 3 negative controls.
Web link
http://www.miltenyibiotec.com Contributor(s)
Gerstmayer B , Bosio A Submission date
Apr 14, 2009
Last update date
Oct 27, 2009
Contact name
Ute Bissels
E-mail(s)
ute.bissels@miltenyibiotec.de Organization name
Miltenyi Biotec GmbH
Department
R&D
Street address
Friedrich-Ebert-Str. 68
City
Bergisch Gladbach
State/province
NRW
ZIP/Postal code
51429
Country
Germany
Samples (2)
GSM397600 , GSM397601 Series (1)
Data table header descriptions ID miRNA_ID_LIST Stem-loop_acc
miRNA_ACC Mature_acc
NAME Name
miRNA_ID miRNA identifiers
miltenyi_id cDNA identifier at Miltenyi Biotec GmbH
SPOT_ID spot identifiers
Data table ID miRNA_ID_LIST miRNA_ACC NAME miRNA_ID miltenyi_id SPOT_ID 1
MI0000558 , MI0000829 , MI0000063 , MI0005453 , MI0005351 , MI0001172 MIMAT0000522 , MIMAT0000775 , MIMAT0000063 , MIMAT0004331 , MIMAT0004162 , MIMAT0001102 LET-7B
LET-7B 35816
2
MI0000442 , MI0000256 , MI0000891 , MI0005063 , MI0005287 , MI0002413 , MI0001277 , MI0001280 MIMAT0000827 , MIMAT0000246 , MIMAT0000421 , MIMAT0003849 , MIMAT0004101 , MIMAT0002119 , MIMAT0001190 MIR-122
MIR-122 35826
3
MI0000897 , MI0000446 , MI0000470 , MI0000152 , MI0000725 , MI0000896 , MI0002510 , MI0002575 , MI0004753 , MI0005457 , MI0002509 , MI0002574 , MI0002514 , MI0002579 , MI0002517 , MI0002581 , MI0005291 , MI0005292 , MI0002511 , MI0002513 , MI0002578 , MI0002512 , MI0002577 , MI MIMAT0000830 , MIMAT0000136 , MIMAT0000423 , MIMAT0002221 , MIMAT0003539 , MIMAT0002220 , MIMAT0002225 , MIMAT0002228 , MIMAT0004103 , MIMAT0002222 , MIMAT0002224 , MIMAT0002223 , MIMAT0002226 , MIMAT0002120 , MIMAT0001105 MIR-125B
MIR-125B 35830
4
MI0000450 , MI0000451 , MI0000159 , MI0000820 , MI0000906 MIMAT0000427 , MIMAT0000145 , MIMAT0000839 MIR-133A
MIR-133A 35840
5
MI0000459 , MI0000257 , MI0000916 MIMAT0000849 , MIMAT0000247 , MIMAT0000435 MIR-143
MIR-143 35852
6
MI0000070 , MI0000115 , MI0000565 , MI0000566 , MI0000844 , MI0002946 , MI0004739 , MI0002948 , MI0002958 , MI0002950 , MI0005334 , MI0002954 , MI0002962 , MI0002960 , MI0002952 , MI0007589 MIMAT0000785 , MIMAT0000527 , MIMAT0000069 , MIMAT0002639 , MIMAT0003525 , MIMAT0002641 , MIMAT0002651 , MIMAT0002643 , MIMAT0004145 , MIMAT0002647 , MIMAT0002655 , MIMAT0002653 , MIMAT0002645 MIR-16
MIR-16 35866
7
MI0000488 , MI0000732 , MI0000236 , MI0000733 , MI0000937 , MI0000938 , MI0003034 , MI0003030 , MI0003024 , MI0003032 , MI0003026 , MI0003028 , MI0001202 , MI0007657 MIMAT0000869 , MIMAT0000224 , MIMAT0000460 , MIMAT0002737 , MIMAT0002733 , MIMAT0002727 , MIMAT0002735 , MIMAT0002729 , MIMAT0002731 , MIMAT0001133 MIR-194
MIR-194 35878
8
MI0000079 , MI0000571 , MI0000852 , MI0003038 , MI0005042 , MI0003035 , MI0003043 , MI0003051 , MI0003056 , MI0005363 , MI0003040 , MI0003048 , MI0003045 , MI0003054 , MI0002427 MIMAT0000792 , MIMAT0000532 , MIMAT0000078 , MIMAT0002741 , MIMAT0003827 , MIMAT0002738 , MIMAT0002745 , MIMAT0002752 , MIMAT0002756 , MIMAT0004173 , MIMAT0002743 , MIMAT0002749 , MIMAT0002747 , MIMAT0002754 , MIMAT0002133 MIR-23A
MIR-23A 35887
9
MI0000087 , MI0000576 , MI0000863 MIMAT0000086 , MIMAT0000535 , MIMAT0000802 MIR-29A
MIR-29A 35895
10
MI0000441 , MI0000145 , MI0000868 , MI0002507 , MI0004761 , MI0002504 , MI0002505 , MI0002502 , MI0002506 , MI0002508 , MI0002503 , MI0001199 MIMAT0000806 , MIMAT0000130 , MIMAT0000420 , MIMAT0002218 , MIMAT0003547 , MIMAT0002215 , MIMAT0002216 , MIMAT0002213 , MIMAT0002217 , MIMAT0002219 , MIMAT0002214 , MIMAT0001130 MIR-30B
MIR-30B 35899
11
35924
UNKNOWN
12
35931
UNKNOWN
13
MIRNA-NEGCON1
35934
MIRNA-NEGCON1
14
MIR-POSCON1-TARGET
35935
MIR-POSCON1-TARGET
15
MIR-POSCON2-TARGET
35936
MIR-POSCON2-TARGET
16
MIR-POSCON3-TARGET
35937
MIR-POSCON3-TARGET
17
MIR-POSCON4-TARGET
35938
MIR-POSCON4-TARGET
18
MIR-POSCON5-TARGET
35939
MIR-POSCON5-TARGET
19
GFP CONTROL SEQUENCE
35940
GFP CONTROL SEQUENCE
20
TRNA-CONTROL-SEQUENCE
35941
TRNA-CONTROL-SEQUENCE
117 5 Kbytes .Supplementary data files not provided
