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Status
Public on Jun 26, 2009
Title
Ryerson University E-coli O157:H7 MWG 7K array version 1
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Escherichia coli Manufacturer
Public Health Agency of Canada
Manufacture protocol
Corning Ultra-Gap II slides (Corning, Acton, MA) were spotted with the MWG E. coli O157:H7 array set (MWG Biotech). The MWG array consists of 6167 50-mer oligonucleotides covering the genomes of E. coli K-12 (MG1655) and E. coli O157:H7 strains Sakai (RIMD 0509952) and EDL933 (ATCC700927), with each gene spotted twice.
Catalog number
oligos from Ocimum Biosolutions (catalogue # 2340-100000)
Support
glass
Coating
other
Contributor(s)
House B , Prayitano N , Mair RW , Kus JV , Que L , Gannon V , Cvitkovitch DG , Barnett Foster D Submission date
Jun 26, 2008
Last update date
Jun 26, 2009
Contact name
Debora Foster
E-mail(s)
dfoster@gwemail.ryerson.ca Phone
416-979-5000
Fax
416-979-5044
Organization name
Ryerson University
Department
Chemistry and Biology
Street address
350 Victoria Street
City
Toronto
State/province
Ontario
ZIP/Postal code
M5B 2K3
Country
Canada
Samples (4)
GSM352908 , GSM352910 , GSM352911 , GSM544430 Series (2)
GSE14069 Microarray Analysis of Acid-Stress Induced Changes in EHEC O157:H7
GSE22060 Bile Salts Influence Virulence Properties of E. coli O157:H7
Data table header descriptions ID ORF Gene name proposed gene names
Gene Description proposed function of encoded protein
MWG ID manufacturer's oligo identification
Data table ID ORF Gene name Gene Description MWG ID B0001
B0001 thrL [B]
thr operon leader peptide
mwgecov2#0001
B0017
B0017 yi82_1 [B]
IS186 and IS421 hypothetical protein
mwgecov2#0017
B0024
B0024 b0024 [BEZ]
orf, hypothetical protein
mwgecov2#4166
B0025
B0025 ribF [BEZ]
putative regulator
mwgecov2#0022
B0026
B0026 ileS [BEZ]
isoleucine tRNA synthetase
mwgecov2#0023
B0027
B0027 lspA [BEZ]
prolipoprotein signal peptidase (SPase II)
mwgecov2#0024
B0033
B0033 carB [BEZ]
carbamoyl-phosphate synthase large subunit
mwgecov2#0030
B0034
B0034 caiF [BEZ]
transcriptional regulator of cai operon
mwgecov2#0031
B0035
B0035 caiE [BEZ]
possible synthesis of cofactor for carnitine racemase and dehydratase
mwgecov2#0032
B0042
B0042 fixB [BEZ]
probable flavoprotein subunit, carnitine metabolism
mwgecov2#0039
B0043
B0043 fixC [BEZ]
flavoprotein, electron transport
mwgecov2#0040
B0044
B0044 fixX [BEZ]
putative ferredoxin
mwgecov2#4167
B0057
B0057 yabQ [B]
orf, hypothetical protein
mwgecov2#0053
B0059
B0059 hepA [BEZ]
probable ATP-dependent RNA helicase
mwgecov2#0055
B0069
B0069 yabN [BEZ]
putative transport protein
mwgecov2#0065
B0070
B0070 yabM [B]
putative transport protein
mwgecov2#0066
B0076
B0076 leuO [B]
probable transcriptional activator for leuABCD operon
mwgecov2#0072
B0077
B0077 ilvI [BEZ]
acetolactate synthase III, valine sensitive, large subunit
mwgecov2#0073
B0078
B0078 ilvH [BEZ]
acetolactate synthase III, valine sensitive, small subunit
mwgecov2#0074
B0079
B0079 fruL [BEZ]
fruR leader peptide
mwgecov2#0075
6176 422 Kbytes .Supplementary file Size Download File type/resource GPL7001_MWG_O157_array_platform_duplicates.txt.gz
142.2 Kb
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